sulfophobococcus_medium

Overview

External ontology

TOGO:M244 — Sulfophobococcus Medium

Applications

• Microbial cultivation

pH

7.6

Notes

Source: https://togomedium.org/medium/M244 Original source: JCM - JCM_M252 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=252 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The exact recipe specifies albumin, not LB broth. Remove only the three reviewed LB supplier nodes and restore albumin at its source-defined preparation stage. Genuine yeast extract and unrelated annotations are preserved. Withdraw the unsupported high_metal classification, not replace it with false. Its schema definition requires total metal concentration >=1049.84 mM. Source quantities include milligram batch inputs, one liter of water, and later stocks; a final-volume denominator and all molecular-form conversions are not established. The former g/L fields cannot support this classification.

Composition

flowchart LR
    CultureMech_009029["sulfophobococcus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009029 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009029 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_009029 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_Resazurin["Resazurin"]
    CultureMech_009029 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_009029 --> ing4_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_7H2O -.-> CHEBI_75836
    ing5_KCl["KCl"]
    CultureMech_009029 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_ZnCl2["ZnCl2"]
    CultureMech_009029 --> ing6_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing6_ZnCl2 -.-> CHEBI_49976
    ing7_Na2CO3["Na2CO3"]
    CultureMech_009029 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_MnSO4_xH2O["MnSO4・xH2O"]
    CultureMech_009029 --> ing8_MnSO4_xH2O
    CHEBI_86360["CHEBI:86360"]
    ing8_MnSO4_xH2O -.-> CHEBI_86360
    ing9_Glycine["Glycine"]
    CultureMech_009029 --> ing9_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing9_Glycine -.-> CHEBI_15428
    ing10_Na2B4O7_10H2O["Na2B4O7・10H2O"]
    CultureMech_009029 --> ing10_Na2B4O7_10H2O
    CHEBI_131366["CHEBI:131366"]
    ing10_Na2B4O7_10H2O -.-> CHEBI_131366
    ing11_EDTA_2Na["EDTA・2Na"]
    CultureMech_009029 --> ing11_EDTA_2Na
    CHEBI_64734["CHEBI:64734"]
    ing11_EDTA_2Na -.-> CHEBI_64734
    ing12_N2["N2"]
    CultureMech_009029 --> ing12_N2
    CHEBI_17997["CHEBI:17997"]
    ing12_N2 -.-> CHEBI_17997

Ingredients (13)

Preparation

1. Mix components except yeast extract, bovine serum albumin and Na2S·9H2O.
2. Bring to a boil for 5-10 sec and flush with N2.
3. Adjust pH to 7.6 at 80-90C.
4. After cooling to room temperature, dispense the medium into serum bottles under a N2 atmosphere, seal with butyl rubber stoppers and autoclave.
5. Separately autoclave yeast extract as a 10% solution and neutralized Na2S·9H2O as a 5% solution under a N2 atmosphere.
6. Filter-sterilize bovine serum albumin as a 10% (w/v) solution under a N2 atmosphere.
7. Before inoculation, aseptically and anaerobically add the yeast extract, bovine serum albumin and neutralized Na2S·9H2O solutions.

Solutions

Target organisms

No target organisms are recorded.

Provenance