Overview
- External ontology
- TOGO:M2473 — PPLO broth with bovine serum
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2473 Original URL: https://www.atcc.org/~/media/ED4787F5B1F547798D9ADD912ADFEED1.ashx
Composition
flowchart LR
CultureMech_009049["pplo_broth_with_bovine_serum"]
ing0_Deionized_water["Deionized water"]
CultureMech_009049 --> ing0_Deionized_water
ing1_PPLO_Broth_Base_w_o_CV__Mycoplasma_Broth_Base___BD_211458_["PPLO Broth Base w/o CV (Mycoplasma Broth Base) (BD 211458)"]
CultureMech_009049 --> ing1_PPLO_Broth_Base_w_o_CV__Mycoplasma_Broth_Base___BD_211458_
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Deionized water | 865 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component | |
| 2 | PPLO Broth Base w/o CV (Mycoplasma Broth Base) (BD 211458) | 21 G_PER_L |
Solutions
Phenol red solution, 1% stock solution
Dose: 2 G_PER_L
Role: pH indicator; Properties: Defined component, Solution, Organic compound
Glucose (20% solution)*
Dose: 25 G_PER_L
Role: Carbon source; Properties: Defined component, Solution, Organic compound
Bovine serum*
Dose: 10 G_PER_L
Role: Nutrient source
Yeast Extract Solution (GIBCO 18180)
Dose: 100 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pplo_broth_with_bovine_serum (CultureMech:009049)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.556287Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2473 |
| 2026-03-09T08:04:38.048140+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:20:02.224795Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009049 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T19:46:20.994763+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PPLO broth with bovine serum" to "pplo_broth_with_bovine_serum" for consistent matching |
| 2026-05-16T09:04:29.387292+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-08-06T03:05:53.435821+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4a36e519aa0bc64ae41ffe94c883624b16f091728bb14e7725fadd84b1d26ce0 |