Overview
- External ontology
- TOGO:M2473 — PPLO broth with bovine serum
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- TOGO M2473 cites ATCC Medium 1106; ATCC Medium 1106 defines PPLO broth with bovine serum as PPLO Broth Base w/o CV, phenol red solution, and deionized water adjusted to pH 7.5, then supplemented with glucose, bovine serum, and yeast extract.
Composition
flowchart LR
CultureMech_009049["pplo_broth_with_bovine_serum"]
ing0_PPLO_Broth_Base_w_o_CV__Mycoplasma_Broth_Base___BD_211458_["PPLO Broth Base w/o CV (Mycoplasma Broth Base) (BD 211458)"]
CultureMech_009049 --> ing0_PPLO_Broth_Base_w_o_CV__Mycoplasma_Broth_Base___BD_211458_
ing1_DI_Water["DI Water"]
CultureMech_009049 --> ing1_DI_Water
CHEBI_15377["CHEBI:15377"]
ing1_DI_Water -.-> CHEBI_15377
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | PPLO Broth Base w/o CV (Mycoplasma Broth Base) (BD 211458) | 21.0 G_PER_L | ATCC Medium 1106 lists 21.0 g PPLO Broth Base w/o CV (Mycoplasma Broth Base) from BD. | |
| 2 |
DI Water
H2O 18.015 g/mol
|
865.0 ML_PER_L |
CHEBI:15377
water
|
ATCC Medium 1106 lists 865.0 mL deionized water. |
Preparation
- Dissolve PPLO Broth Base w/o CV, 1% phenol red solution, and deionized water.
- Adjust pH to 7.5.
- Autoclave the base medium.
- Aseptically add filter-sterilized 20% glucose solution, bovine serum, and Yeast Extract Solution.
Solutions
Phenol red solution, 1% stock solution
Dose: 2.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Phenol red | 1 PERCENT_W_V | ATCC Medium 1106 identifies this as a 1% stock solution. |
ATCC Medium 1106 lists 2.0 mL 1% phenol red solution.
Glucose (20% solution)
Dose: 25.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Glucose | 20 PERCENT_W_V | ATCC Medium 1106 identifies this as a 20% stock solution. |
ATCC Medium 1106 aseptically adds 25.0 mL 20% glucose solution.
Bovine serum
Dose: 10.0 ML_PER_L
ATCC Medium 1106 aseptically adds 10.0 mL filter-sterilized bovine serum.
Yeast Extract Solution (GIBCO 18180)
Dose: 100.0 ML_PER_L
ATCC Medium 1106 aseptically adds 100.0 mL Yeast Extract Solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.556287Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2473 |
| 2026-03-09T08:04:38.048140+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:20:02.224795Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009049 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T19:46:20.994763+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PPLO broth with bovine serum" to "pplo_broth_with_bovine_serum" for consistent matching |
| 2026-05-16T09:04:29.387292+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-07T00:00:00-07:00 | repair_togo_exact_score25.py | RESOLVED_TOGO_EXACT_SCORE25_GRAPH | TOGO M2473 cites ATCC Medium 1106; ATCC Medium 1106 defines PPLO broth with bovine serum as PPLO Broth Base w/o CV, phenol red solution, and deionized water adjusted to pH 7.5, then supplemented with glucose, bovine serum, and yeast extract. |