Overview
- External ontology
- TOGO:M2578 — Spiroplasma Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2578 Original URL: https://www.atcc.org/~/media/D078A95CAFED4ADE877F47632F4E2EB5.ashx
Composition
flowchart LR
CultureMech_009147["spiroplasma_medium"]
ing0_DI_Water["DI Water"]
CultureMech_009147 --> ing0_DI_Water
ing1_Agar["Agar"]
CultureMech_009147 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
ing2_Tryptone__Difco_["Tryptone (Difco)"]
CultureMech_009147 --> ing2_Tryptone__Difco_
ing3_PPLO_Broth_w_o__Crystal_Violet__Difco_["PPLO Broth w/o Crystal Violet (Difco)"]
CultureMech_009147 --> ing3_PPLO_Broth_w_o__Crystal_Violet__Difco_
ing4_Fetal_bovine_serum__heat_inactivated_["Fetal bovine serum (heat-inactivated)"]
CultureMech_009147 --> ing4_Fetal_bovine_serum__heat_inactivated_
ing5_TC_Yeastolate__2__soln____BD_255772_["TC Yeastolate (2% soln.) (BD 255772)"]
CultureMech_009147 --> ing5_TC_Yeastolate__2__soln____BD_255772_
ing6_Glucose["Glucose"]
CultureMech_009147 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Mycoplasma_Growth_Supplement__CMRL_1066__10X_["Mycoplasma Growth Supplement (CMRL 1066)(10X)"]
CultureMech_009147 --> ing7_Mycoplasma_Growth_Supplement__CMRL_1066__10X_
ing8_Distilled_water["Distilled water"]
CultureMech_009147 --> ing8_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing8_Distilled_water -.-> CHEBI_15377
ing9_CMRL_1066__ATCC_20_2206_["CMRL-1066 (ATCC 20-2206)"]
CultureMech_009147 --> ing9_CMRL_1066__ATCC_20_2206_
ing10_Distilled_deionized_water["Distilled deionized water"]
CultureMech_009147 --> ing10_Distilled_deionized_water
ing11_Bakers__yeast__live__pressed__starch_free_["Bakers’ yeast (live, pressed, starch-free)"]
CultureMech_009147 --> ing11_Bakers__yeast__live__pressed__starch_free_
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | DI Water | 700.0 G_PER_L | Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 2 duplicates: 500.0, 200.0] | |
| 2 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 3 | Tryptone (Difco) | 10 G_PER_L | Properties: Undefined component | |
| 4 | PPLO Broth w/o Crystal Violet (Difco) | 11 G_PER_L | Properties: Complex component, Undefined component | |
| 5 | Fetal bovine serum (heat-inactivated) | 170 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 | TC Yeastolate (2% soln.) (BD 255772) | 100 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 8 | Mycoplasma Growth Supplement (CMRL 1066)(10X) | 50 G_PER_L | ||
| 9 |
Distilled water
H2O 18.015 g/mol
|
100 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 10 | CMRL-1066 (ATCC 20-2206) | 98 G_PER_L | Properties: Complex component, Defined component | |
| 11 | Distilled deionized water | 1 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component | |
| 12 | Bakers’ yeast (live, pressed, starch-free) | 250 G_PER_L | Role: Nutrient source; Properties: Undefined component |
Solutions
Phenol red solution (0.1% soln.)
Dose: 20 G_PER_L
Role: pH indicator; Properties: Organic compound, Solution, Defined component
Yeast Extract Solution
Dose: 35 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- spiroplasma_medium (CultureMech:009147)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.783945Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2578 |
| 2026-01-28T19:48:10.586736Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:17.339897+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:44.876633Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:03.270821Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009147 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.000497+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Spiroplasma Medium" to "spiroplasma_medium" for consistent matching |
| 2026-05-16T09:04:32.113050+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:46.433087+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:56.159092+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 23e4c1eabb0a9f917a000c359695900a0926e46fe524477dbad2aec77580f184 |