spiroplasma_medium

Overview

External ontology

TOGO:M2578 — Spiroplasma Medium

Applications

• Microbial cultivation

pH range

{'min': 7.25, 'max': 7.45}

Notes

TOGO M2578 defines Spiroplasma Medium with a basal medium containing 500 ml DI Water, 15 g optional Agar, 10 g Tryptone, and 11 g PPLO Broth without crystal violet. Its additive solution supplies 170 ml heat-inactivated fetal bovine serum, 20 ml 0.1% Phenol red solution, 100 ml 2% TC Yeastolate, 200 ml DI Water, 5 g Glucose, 50 ml Mycoplasma Growth Supplement made from 98 g CMRL-1066 in 1.0 L water, and 35 ml Yeast Extract Solution made from 250 g baker's yeast in 1.0 L water. The basal medium is adjusted to pH 7.3-7.4, autoclaved at 121 C for 15 minutes, combined with the filter-sterilized additive solution, and readjusted if needed to pH 7.35 +/- 0.1.

Composition

flowchart LR
    CultureMech_009147["spiroplasma_medium"]
    ing0_DI_Water["DI Water"]
    CultureMech_009147 --> ing0_DI_Water
    CHEBI_15377["CHEBI:15377"]
    ing0_DI_Water -.-> CHEBI_15377
    ing1_Agar["Agar"]
    CultureMech_009147 --> ing1_Agar
    CHEBI_2509["CHEBI:2509"]
    ing1_Agar -.-> CHEBI_2509
    ing2_Tryptone__Difco_["Tryptone (Difco)"]
    CultureMech_009147 --> ing2_Tryptone__Difco_
    ing3_PPLO_Broth_w_o__Crystal_Violet__Difco_["PPLO Broth w/o  Crystal Violet (Difco)"]
    CultureMech_009147 --> ing3_PPLO_Broth_w_o__Crystal_Violet__Difco_
    ing4_Fetal_bovine_serum__heat_inactivated_["Fetal bovine serum (heat-inactivated)"]
    CultureMech_009147 --> ing4_Fetal_bovine_serum__heat_inactivated_
    ing5_Glucose["Glucose"]
    CultureMech_009147 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234

Ingredients (6)

Preparation

1. Mix DI Water, Agar if required, Tryptone, and PPLO Broth without crystal violet as the basal medium.
2. Adjust the basal medium to pH 7.3-7.4.
3. Autoclave the basal medium at 121 C for 15 minutes, then cool to 50-55 C in a water bath if agar is used.
4. Prepare the additive solution from heat-inactivated fetal bovine serum, Phenol red solution, TC Yeastolate, DI Water, Glucose, Mycoplasma Growth Supplement, and Yeast Extract Solution, then filter-sterilize it.
5. Combine the filter-sterilized additive solution with the basal medium and, if needed, readjust the pH to 7.35 +/- 0.1.

Solutions

Target organisms

No target organisms are recorded.

Provenance