desulfurobacterium_medium (CultureMech:009155)

Overview

External ontology

TOGO:M2587 — Desulfurobacterium Medium

Applications

Microbial cultivation

High-metal flag

True

Notes

Source: https://togomedium.org/medium/M2587 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium829.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_009155["desulfurobacterium_medium"]
    ing0_Sodium_resazurin__0_1__w_v_["Sodium resazurin (0.1% w/v)"]
    CultureMech_009155 --> ing0_Sodium_resazurin__0_1__w_v_
    ing1_Distilled_water["Distilled water"]
    CultureMech_009155 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_KH2PO4["KH2PO4"]
    CultureMech_009155 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_009155 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Na2CO3["Na2CO3"]
    CultureMech_009155 --> ing4_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing4_Na2CO3 -.-> CHEBI_29377
    ing5_Sulfur__powdered["Sulfur, powdered"]
    CultureMech_009155 --> ing5_Sulfur__powdered
    ing6_MES__2__N_morpholino__ethane_sulfonic_acid_["MES [2-(N-morpholino) ethane sulfonic acid]"]
    CultureMech_009155 --> ing6_MES__2__N_morpholino__ethane_sulfonic_acid_
    CHEBI_39005["CHEBI:39005"]
    ing6_MES__2__N_morpholino__ethane_sulfonic_acid_ -.-> CHEBI_39005
    ing7_Sea_Salts__SIGMA_["Sea Salts (SIGMA)"]
    CultureMech_009155 --> ing7_Sea_Salts__SIGMA_
    ing8_Growth_stimulating_factors["Growth-stimulating factors"]
    CultureMech_009155 --> ing8_Growth_stimulating_factors
    ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009155 --> ing9_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing9_Carbon_dioxide_gas -.-> CHEBI_16526
    ing10_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009155 --> ing10_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing10_Hydrogen_gas -.-> CHEBI_18276
    ing11_Valeric_acid["Valeric acid"]
    CultureMech_009155 --> ing11_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing11_Valeric_acid -.-> CHEBI_17418
    ing12_Tryptone["Tryptone"]
    CultureMech_009155 --> ing12_Tryptone
    ing13_Yeast_extract["Yeast extract"]
    CultureMech_009155 --> ing13_Yeast_extract
    ing14_Sodium_chloride["Sodium chloride"]
    CultureMech_009155 --> ing14_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing14_Sodium_chloride -.-> CHEBI_26710
    ing15_Isobutyric_acid["Isobutyric acid"]
    CultureMech_009155 --> ing15_Isobutyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing15_Isobutyric_acid -.-> CHEBI_16135
    ing16_Caproic_acid["Caproic acid"]
    CultureMech_009155 --> ing16_Caproic_acid
    CHEBI_30776["CHEBI:30776"]
    ing16_Caproic_acid -.-> CHEBI_30776
    ing17_Succinic_acid["Succinic acid"]
    CultureMech_009155 --> ing17_Succinic_acid
    CHEBI_15741["CHEBI:15741"]
    ing17_Succinic_acid -.-> CHEBI_15741
    ing18_N2_gas["N2 gas"]
    CultureMech_009155 --> ing18_N2_gas
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_009155 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_H3BO3["H3BO3"]
    CultureMech_009155 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_009155 --> ing21_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing21_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing22_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_009155 --> ing22_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing22_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_009155 --> ing23_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing24_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_009155 --> ing24_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing24_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing25_ZnCl2["ZnCl2"]
    CultureMech_009155 --> ing25_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing25_ZnCl2 -.-> CHEBI_49976
    ing26_HCl__25___7_7_M_["HCl (25%; 7.7 M)"]
    CultureMech_009155 --> ing26_HCl__25___7_7_M_
    ing27_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_009155 --> ing27_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing27_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing28_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_009155 --> ing28_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing28_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing29_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_009155 --> ing29_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing29_Na2WO4_x_2_H2O -.-> CHEBI_63939
    CultureMech_009155 --> more["...10 more"]

Ingredients (40)

#	Ingredient	Concentration	Ontology	Notes
1	Sodium resazurin (0.1% w/v)	0.5 G_PER_L		Role: pH dependent redox indicator; Properties: Defined component, Solution
2	Distilled water H2O 18.015 g/mol	5990.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 6 duplicates: 1000.0, 1000.0, 990.0, 1000.0, 1000.0, 1000.0]
3	KH2PO4 H2O4P.K 136.084 g/mol	0.35 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
4	NH4Cl Cl.H4N 53.492 g/mol	1 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
5	Na2CO3 CO3.2Na 105.988 g/mol	0.5 G_PER_L	CHEBI:29377 sodium carbonate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6	Sulfur, powdered	10 G_PER_L		Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7	MES [2-(N-morpholino) ethane sulfonic acid] C6H13NO4S 195.24 g/mol	1.95 G_PER_L	CHEBI:39005 2-(N-morpholino)ethanesulfonic acid	Role: Buffer; Properties: Organic compound, Defined component, Simple component
8	Sea Salts (SIGMA)	30 G_PER_L		Role: Mineral source; Properties: Defined component, Complex component
9	Growth-stimulating factors	1 G_PER_L
10	Carbon dioxide gas CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Properties: ガス
11	Hydrogen gas H2 2.016 g/mol	variable VARIABLE	CHEBI:18276 dihydrogen	Properties: Gas
12	Valeric acid C5H10O2 102.133 g/mol	5 G_PER_L	CHEBI:17418 valeric acid	Role: Carbon source; Properties: Organic compound, Defined component, Simple component
13	Tryptone Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)	10.0 G_PER_L	MICRO:0000182 (source link unavailable) tryptone	Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
14	Yeast extract Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)	5.0 G_PER_L	FOODON:03315426 yeast extract	Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
15	Sodium chloride Cl.Na 58.443 g/mol Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)	10.0 G_PER_L	CHEBI:26710 sodium chloride	Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
16	Isobutyric acid C4H8O2 88.106 g/mol	5 G_PER_L	CHEBI:16135 isobutyric acid	Role: Carbon source; Properties: Organic compound, Defined component, Simple component
17	Caproic acid C6H12O2 116.16 g/mol	2 G_PER_L	CHEBI:30776 hexanoic acid	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
18	Succinic acid C4H6O4 118.088 g/mol	6 G_PER_L	CHEBI:15741 succinic acid	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
19	N2 gas	variable VARIABLE		Properties: ガス
20	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	36 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
21	H3BO3 H3BO3 61.833 g/mol	6 G_PER_L	CHEBI:33118 boric acid	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	100 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
23	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	190 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
24	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	24 G_PER_L	CHEBI:34887 nickel dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
25	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	2 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
26	ZnCl2 2Cl.Zn 136.296 g/mol	70 G_PER_L	CHEBI:49976 zinc dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
27	HCl (25%; 7.7 M)	10 G_PER_L		Role: Solvating media; Properties: Inorganic compound, Defined component, Solution
28	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	1.5 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
29	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	3 G_PER_L	CHEBI:131361 disodium selenite pentahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
30	Na2WO4 x 2 H2O 2H2O.2Na.O4W 329.846 g/mol	4 G_PER_L	CHEBI:63939 sodium tungstate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
31	NaOH HO.Na 39.997 g/mol	0.5 G_PER_L	CHEBI:32145 sodium hydroxide	Properties: Defined component, Inorganic compound, Simple component
32	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	80 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
33	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	100 G_PER_L	CHEBI:31345 Calcium pantothenate	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
34	Pyridoxine hydrochloride C8H11NO3.HCl 205.641 g/mol	300 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
35	Vitamin B12	100 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
36	Nicotinic acid C6H5NO2 123.111 g/mol	200 G_PER_L	CHEBI:15940 nicotinic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
37	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	200 G_PER_L	CHEBI:132751 thiamine hydrochloride dihydrate	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
38	D(+)-Biotin C10H16N2O3S 244.316 g/mol	20 G_PER_L	CHEBI:15956 biotin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
39	NaHCO3 CHO3.Na 84.006 g/mol	50 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
40	Na2S2O4 2Na.O4S2 174.11 g/mol	50 G_PER_L	CHEBI:66870 sodium dithionite	Properties: Defined component, Simple component, Inorganic compound

Solutions

Na-dithionite solution (5% w/v)

Dose: 1 G_PER_L

See mediadive_6262_Na-dithionite_solution_5_w_v.yaml for composition

Trace element solution SL-10

Dose: 1 G_PER_L

See mediadive_4178_Trace_element_solution_SL-10.yaml for composition

Selenite-tungstate solution

Dose: 1 G_PER_L

See mediadive_5543_Selenite-tungstate_solution.yaml for composition

Seven vitamins solution

Dose: 1 G_PER_L

See mediadive_4611_Seven_vitamins_solution.yaml for composition

NaOH solution

Dose: variable VARIABLE

Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TOGO_M2587_Desulfurobacterium_Medium (CultureMech:009155)

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.793994Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2587
2026-01-28T19:48:20.142574Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: NaOH solution; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:33.404820+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 5 solution(s) from ingredients
2026-03-13T03:09:02.823776Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 8 duplicate ingredient(s)
2026-03-13T06:20:03.336662Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009155
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:08.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:22.063026+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Desulfurobacterium Medium" to "desulfurobacterium_medium" for consistent matching
2026-05-16T09:03:14.375626+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:37.641264+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:49.298789+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:12.645562+00:00	merge_recipes.py	MERGED_RECIPES	merged 1 source recipe(s) on fingerprint edb237d223bf336ffee92b9a4b59c5323f58d646bcb218d73335c8a1788a0ba9
2026-08-15T20:09:23.657587+00:00	dedupe_identical_ingredients.py	REMOVED_DUPLICATE_INGREDIENT_ROWS	Removed 3 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Sodium chloride, Tryptone, Yeast extract.