desulfurobacterium_medium (CultureMech:009155)

Overview

External ontology

TOGO:M2587 — Desulfurobacterium Medium

Applications

Microbial cultivation

pH

6.0

Notes

Source: https://togomedium.org/medium/M2587 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium829.pdf Superseded historical provenance, not a current formulation claim: Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. TOGO M2587 is a distinct captured source version. It specifies a bicarbonate-based dithionite stock and no final-medium denominator; current DSMZ829's NaOH carrier and reported 1005 ml final volume must not silently replace these assertions. External same-name stock references are withdrawn unless exact composition/version identity is established. The current DSMZ829 dithionite carrier is 0.50 ml of 1 N NaOH, unlike TOGO's bicarbonate stock. This comparison does not replace the source-specific TOGO formulation. The TOGO dithionite section includes a hydrogen-gas item, while its preparation text specifies 80% N2 and 20% CO2. Preserve that upstream discrepancy; do not infer an amount or silently select one gas formulation. Full exact-source recipe and stock/preparation tables were inspected. Reconstructed nodes retain explicit source scope; complete prior ingredient/stock assertions, including copied LB mappings and stage conflations, are archived in append-only history. A title alone is not the basis for removal. Unchanged annotations are preserved, not recertified; no new organism-growth association is added. Full exact-source recipe and stock/preparation tables were inspected. Reconstructed nodes retain explicit source scope; complete prior ingredient/stock assertions, including copied LB mappings and stage conflations, are archived in append-only history. A title alone is not the basis for removal. Unchanged annotations are preserved, not recertified; no new organism-growth association is added.

Composition

flowchart LR
    CultureMech_009155["desulfurobacterium_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009155 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_KH2PO4["KH2PO4"]
    CultureMech_009155 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_009155 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_Na2CO3["Na2CO3"]
    CultureMech_009155 --> ing3_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing3_Na2CO3 -.-> CHEBI_29377
    ing4_Sulfur__powdered["Sulfur, powdered"]
    CultureMech_009155 --> ing4_Sulfur__powdered
    ing5_MES__2__N_morpholino__ethane_sulfonic_acid_["MES [2-(N-morpholino) ethane sulfonic acid]"]
    CultureMech_009155 --> ing5_MES__2__N_morpholino__ethane_sulfonic_acid_
    CHEBI_39005["CHEBI:39005"]
    ing5_MES__2__N_morpholino__ethane_sulfonic_acid_ -.-> CHEBI_39005
    ing6_Sea_Salts__SIGMA_["Sea Salts (SIGMA)"]
    CultureMech_009155 --> ing6_Sea_Salts__SIGMA_
    ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009155 --> ing7_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing7_Carbon_dioxide_gas -.-> CHEBI_16526
    ing8_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009155 --> ing8_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing8_Hydrogen_gas -.-> CHEBI_18276

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1 L	CHEBI:15377 water	Superseded flattening history, not a current quantity: Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 6 duplicates: 1000.0, 1000.0, 990.0, 1000.0, 1000.0, 1000.0] Source preparation input 1000 ml = 1 L, not grams or a final-volume denominator. Keep each stage's water/carrier separate.
2	KH2PO4 H2O4P.K 136.084 g/mol		CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Exact source batch input 0.35 g, retained in notes. This TOGO stage has no explicit final-volume denominator; water input and the differently versioned DSMZ heading do not justify g/L.
3	NH4Cl Cl.H4N 53.492 g/mol		CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component Exact source batch input 1 g, retained in notes. This TOGO stage has no explicit final-volume denominator; water input and the differently versioned DSMZ heading do not justify g/L.
4	Na2CO3 CO3.2Na 105.988 g/mol		CHEBI:29377 sodium carbonate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Exact source batch input 0.5 g, retained in notes. This TOGO stage has no explicit final-volume denominator; water input and the differently versioned DSMZ heading do not justify g/L.
5	Sulfur, powdered			Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Exact source batch input 10 g, retained in notes. This TOGO stage has no explicit final-volume denominator; water input and the differently versioned DSMZ heading do not justify g/L.
6	MES [2-(N-morpholino) ethane sulfonic acid] C6H13NO4S 195.24 g/mol		CHEBI:39005 2-(N-morpholino)ethanesulfonic acid	Role: Buffer; Properties: Organic compound, Defined component, Simple component Exact source batch input 1.95 g, retained in notes. This TOGO stage has no explicit final-volume denominator; water input and the differently versioned DSMZ heading do not justify g/L.
7	Sea Salts (SIGMA)			Role: Mineral source; Properties: Defined component, Complex component Exact source batch input 30 g, retained in notes. This TOGO stage has no explicit final-volume denominator; water input and the differently versioned DSMZ heading do not justify g/L.
8	Carbon dioxide gas CO2 44.009 g/mol		CHEBI:16526 carbon dioxide	Properties: ガス The source gives no fixed addition amount. Preserve the stage-specific gas or titration context without a zero, typical dose or liquid concentration.
9	Hydrogen gas H2 2.016 g/mol		CHEBI:18276 dihydrogen	Properties: Gas The source gives no fixed addition amount. Preserve the stage-specific gas or titration context without a zero, typical dose or liquid concentration.

Preparation

1. Dissolve ingredients (except sulfur, carbonate, vitamins, growth-stimulating factors and Na-dithionite), boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere.
Adjust pH to 6.0 and dispense under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials which contain already the appropriate amount of sulfur, only to 30% of their volume to allow for a large head space.
Autoclave at a temperature of 110°C for 20 min! Add vitamins, growth-stimulating factors from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate and Na-dithionite from sterile anoxic stock solutions prepared under 80% N2 and 20% CO2 gas atmosphere.
Vitamins and dithionite are sterilized by filtration. Adjust pH of the complete medium to 6.0, if necessary.
2. After inoculation use 1 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture.

Solutions

Sodium resazurin (0.1% w/v)

Dose: 0.0005 L

Stock composition
Ingredient	Concentration	Notes
Sodium resazurin	0.1 PERCENT_W_V	Explicit 0.1% w/v indicator strength, separate from the 0.50 ml receiving-batch dose.

Receiving-batch dose 0.5 ml = 0.0005 L. This is not g/L or a fixed per-liter dose. Restore the exact TOGO stock version rather than a same-named external stock.