medium_ycfagsc

CultureMech:009196 bacterial COMPLEX LIQUID
Original name: Medium YCFAGSC

Overview

External ontology
TOGO:M2639 — Medium YCFAGSC
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2639

Composition

flowchart LR
    CultureMech_009196["medium_ycfagsc"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009196 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4___7H2O["MgSO4 · 7H2O"]
    CultureMech_009196 --> ing1_MgSO4___7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4___7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_009196 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_009196 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KH2PO4["KH2PO4"]
    CultureMech_009196 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_K2HPO4["K2HPO4"]
    CultureMech_009196 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Resazurin["Resazurin"]
    CultureMech_009196 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_NaHCO3["NaHCO3"]
    CultureMech_009196 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Biotin["Biotin"]
    CultureMech_009196 --> ing8_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_Biotin -.-> CHEBI_15956
    ing9_p_amino_benzoic_acid["p-amino benzoic acid"]
    CultureMech_009196 --> ing9_p_amino_benzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing9_p_amino_benzoic_acid -.-> CHEBI_30753
    ing10_Folic_acid["Folic acid"]
    CultureMech_009196 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Cobalamin["Cobalamin"]
    CultureMech_009196 --> ing11_Cobalamin
    CHEBI_28911["CHEBI:28911"]
    ing11_Cobalamin -.-> CHEBI_28911
    ing12_Riboflavin["Riboflavin"]
    CultureMech_009196 --> ing12_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing12_Riboflavin -.-> CHEBI_17015
    ing13_Starch["Starch"]
    CultureMech_009196 --> ing13_Starch
    CHEBI_28017["CHEBI:28017"]
    ing13_Starch -.-> CHEBI_28017
    ing14_CaCl2["CaCl2"]
    CultureMech_009196 --> ing14_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing14_CaCl2 -.-> CHEBI_3312
    ing15_Cellobiose["Cellobiose"]
    CultureMech_009196 --> ing15_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing15_Cellobiose -.-> CHEBI_17057
    ing16_Thiamine["Thiamine"]
    CultureMech_009196 --> ing16_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing16_Thiamine -.-> CHEBI_18385
    ing17_Hemin["Hemin"]
    CultureMech_009196 --> ing17_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing17_Hemin -.-> CHEBI_50385
    ing18_Cysteine["Cysteine"]
    CultureMech_009196 --> ing18_Cysteine
    CHEBI_15356["CHEBI:15356"]
    ing18_Cysteine -.-> CHEBI_15356
    ing19_Pyridoxamine["Pyridoxamine"]
    CultureMech_009196 --> ing19_Pyridoxamine
    CHEBI_16410["CHEBI:16410"]
    ing19_Pyridoxamine -.-> CHEBI_16410
    ing20_Glucose["Glucose"]
    CultureMech_009196 --> ing20_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing20_Glucose -.-> CHEBI_17234
    ing21_Casitone["Casitone"]
    CultureMech_009196 --> ing21_Casitone
    ing22_CO2["CO2"]
    CultureMech_009196 --> ing22_CO2
    CHEBI_16526["CHEBI:16526"]
    ing22_CO2 -.-> CHEBI_16526

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
100 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2 MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
0.009 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 Yeast extract 0.25 G_PER_L Role: Nutrient source; Properties: Undefined component
4 NaCl
Cl.Na 58.443 g/mol
0.09 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 KH2PO4
H2O4P.K 136.084 g/mol
0.045 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6 K2HPO4
HO4P.2K 174.174 g/mol
0.045 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
7 Resazurin
C12H7NO4 229.191 g/mol
0.1 G_PER_L CHEBI:8806
Resazurin
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
8 NaHCO3
CHO3.Na 84.006 g/mol
0.4 G_PER_L CHEBI:32139
sodium hydrogencarbonate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
9 Biotin
C10H16N2O3S 244.316 g/mol
1 G_PER_L CHEBI:15956
biotin
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
10 p-amino benzoic acid
C7H7NO2 137.138 g/mol
3 G_PER_L CHEBI:30753
4-aminobenzoic acid
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
11 Folic acid
C19H19N7O6 441.404 g/mol
5 G_PER_L CHEBI:27470
folic acid
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
12 Cobalamin
C62H88CoN13O14P 1329.37 g/mol
1 G_PER_L CHEBI:28911
cob(III)alamin
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
13 Riboflavin
C17H20N4O6 376.369 g/mol
5 G_PER_L CHEBI:17015
riboflavin
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14 Starch 0.2 G_PER_L CHEBI:28017
starch
Role: Carbon source; Properties: Organic compound, Defined component, Complex component
15 CaCl2
Ca.2Cl 110.984 g/mol
0.009 G_PER_L CHEBI:3312
calcium dichloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16 Cellobiose
C12H22O11 342.297 g/mol
0.2 G_PER_L CHEBI:17057
cellobiose
Role: Carbon source; Properties: Organic compound, Defined component, Simple component
17 Thiamine
C12H17N4OS 265.362 g/mol
5 G_PER_L CHEBI:18385
thiamine(1+)
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
18 Hemin
C34H32ClFeN4O4 651.952 g/mol
1 G_PER_L CHEBI:50385
hemin
Role: Growth factor; Properties: Organic compound, Defined component, Simple component
19 Cysteine
C3H7NO2S 121.161 g/mol
0.1 G_PER_L CHEBI:15356
cysteine
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
20 Pyridoxamine
C8H12N2O2 168.196 g/mol
15 G_PER_L CHEBI:16410
pyridoxamine
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
21 Glucose
C6H12O6 180.156 g/mol
0.2 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Organic compound, Defined component, Simple component
22 Casitone 1 G_PER_L Properties: Undefined component
23 CO2
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
Properties: Gas

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:56.903240Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2639
2026-01-28T19:48:13.595948Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: CO2
2026-03-13T06:20:03.869581Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009196
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:22.598863+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Medium YCFAGSC" to "medium_ycfagsc" for consistent matching
2026-05-16T09:04:21.168494+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:30.424324+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:22.005706+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:06.555194+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:44.417530+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint e7a359ff1d1bf450cec7bd3d5374bb95c3be2fdbb7d6d036265f56361d938846