Overview
- External ontology
- TOGO:M2639 — Medium YCFAGSC
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2639
Composition
flowchart LR
CultureMech_009196["medium_ycfagsc"]
ing0_Distilled_water["Distilled water"]
CultureMech_009196 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 · 7H2O"]
CultureMech_009196 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009196 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009196 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KH2PO4["KH2PO4"]
CultureMech_009196 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_009196 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_009196 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_NaHCO3["NaHCO3"]
CultureMech_009196 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Biotin["Biotin"]
CultureMech_009196 --> ing8_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_Biotin -.-> CHEBI_15956
ing9_p_amino_benzoic_acid["p-amino benzoic acid"]
CultureMech_009196 --> ing9_p_amino_benzoic_acid
CHEBI_30753["CHEBI:30753"]
ing9_p_amino_benzoic_acid -.-> CHEBI_30753
ing10_Folic_acid["Folic acid"]
CultureMech_009196 --> ing10_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing10_Folic_acid -.-> CHEBI_27470
ing11_Cobalamin["Cobalamin"]
CultureMech_009196 --> ing11_Cobalamin
CHEBI_28911["CHEBI:28911"]
ing11_Cobalamin -.-> CHEBI_28911
ing12_Riboflavin["Riboflavin"]
CultureMech_009196 --> ing12_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing12_Riboflavin -.-> CHEBI_17015
ing13_Starch["Starch"]
CultureMech_009196 --> ing13_Starch
CHEBI_28017["CHEBI:28017"]
ing13_Starch -.-> CHEBI_28017
ing14_CaCl2["CaCl2"]
CultureMech_009196 --> ing14_CaCl2
CHEBI_3312["CHEBI:3312"]
ing14_CaCl2 -.-> CHEBI_3312
ing15_Cellobiose["Cellobiose"]
CultureMech_009196 --> ing15_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing15_Cellobiose -.-> CHEBI_17057
ing16_Thiamine["Thiamine"]
CultureMech_009196 --> ing16_Thiamine
CHEBI_18385["CHEBI:18385"]
ing16_Thiamine -.-> CHEBI_18385
ing17_Hemin["Hemin"]
CultureMech_009196 --> ing17_Hemin
CHEBI_50385["CHEBI:50385"]
ing17_Hemin -.-> CHEBI_50385
ing18_Cysteine["Cysteine"]
CultureMech_009196 --> ing18_Cysteine
CHEBI_15356["CHEBI:15356"]
ing18_Cysteine -.-> CHEBI_15356
ing19_Pyridoxamine["Pyridoxamine"]
CultureMech_009196 --> ing19_Pyridoxamine
CHEBI_16410["CHEBI:16410"]
ing19_Pyridoxamine -.-> CHEBI_16410
ing20_Glucose["Glucose"]
CultureMech_009196 --> ing20_Glucose
CHEBI_17234["CHEBI:17234"]
ing20_Glucose -.-> CHEBI_17234
ing21_Casitone["Casitone"]
CultureMech_009196 --> ing21_Casitone
ing22_CO2["CO2"]
CultureMech_009196 --> ing22_CO2
CHEBI_16526["CHEBI:16526"]
ing22_CO2 -.-> CHEBI_16526
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
100 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.009 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.25 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.09 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.045 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.045 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 10 |
p-amino benzoic acid
C7H7NO2 137.138 g/mol
|
3 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 11 |
Folic acid
C19H19N7O6 441.404 g/mol
|
5 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 12 |
Cobalamin
C62H88CoN13O14P 1329.37 g/mol
|
1 G_PER_L |
CHEBI:28911
cob(III)alamin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 | Starch | 0.2 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Organic compound, Defined component, Complex component |
| 15 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.009 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.2 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 17 |
Thiamine
C12H17N4OS 265.362 g/mol
|
5 G_PER_L |
CHEBI:18385
thiamine(1+)
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
1 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 19 |
Cysteine
C3H7NO2S 121.161 g/mol
|
0.1 G_PER_L |
CHEBI:15356
cysteine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 20 |
Pyridoxamine
C8H12N2O2 168.196 g/mol
|
15 G_PER_L |
CHEBI:16410
pyridoxamine
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Glucose
C6H12O6 180.156 g/mol
|
0.2 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 22 | Casitone | 1 G_PER_L | Properties: Undefined component | |
| 23 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.903240Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2639 |
| 2026-01-28T19:48:13.595948Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2 |
| 2026-03-13T06:20:03.869581Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009196 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.598863+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium YCFAGSC" to "medium_ycfagsc" for consistent matching |
| 2026-05-16T09:04:21.168494+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:30.424324+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:22.005706+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:06.555194+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |