Overview
- External ontology
- TOGO:M2682 — Obsidian Pool fermentor (OPF) medium (modified from M. B. Allen, 1959)
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2682
Composition
flowchart LR
CultureMech_009237["obsidian_pool_fermentor_opf_medium_modified_from_m_b_allen_1959"]
ing0_Na2MoO4_x2H2O__1_mg_ml_["Na2MoO4 x2H2O (1 mg/ml)"]
CultureMech_009237 --> ing0_Na2MoO4_x2H2O__1_mg_ml_
ing1_MnCl2_x4H2O__10_mg_ml_["MnCl2 x4H2O (10 mg/ml)"]
CultureMech_009237 --> ing1_MnCl2_x4H2O__10_mg_ml_
ing2_ZnSO4_x7H2O__10_mg_ml_["ZnSO4 x7H2O (10 mg/ml)"]
CultureMech_009237 --> ing2_ZnSO4_x7H2O__10_mg_ml_
ing3_CuCl2_x2H2O__10_mg_ml_["CuCl2 x2H2O (10 mg/ml)"]
CultureMech_009237 --> ing3_CuCl2_x2H2O__10_mg_ml_
ing4_CoSO4_x7H2O__1_mg_ml_["CoSO4 x7H2O (1 mg/ml)"]
CultureMech_009237 --> ing4_CoSO4_x7H2O__1_mg_ml_
ing5_Na2B4O7_x_10_H2O__25_mg_ml_["Na2B4O7 x 10 H2O (25 mg/ml)"]
CultureMech_009237 --> ing5_Na2B4O7_x_10_H2O__25_mg_ml_
ing6_VOSO4_x5H2O__1_mg_ml_["VOSO4 x5H2O (1 mg/ml)"]
CultureMech_009237 --> ing6_VOSO4_x5H2O__1_mg_ml_
ing7_Distilled_H2O["Distilled H2O"]
CultureMech_009237 --> ing7_Distilled_H2O
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009237 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_Yeast_Extract["Yeast Extract"]
CultureMech_009237 --> ing9_Yeast_Extract
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009237 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
ing11_KH2PO4["KH2PO4"]
CultureMech_009237 --> ing11_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing11_KH2PO4 -.-> CHEBI_63036
ing12_Na2Sx6H2O["Na2Sx6H2O"]
CultureMech_009237 --> ing12_Na2Sx6H2O
ing13__NH4_2SO4["(NH4)2SO4"]
CultureMech_009237 --> ing13__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing13__NH4_2SO4 -.-> CHEBI_62946
ing14_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_009237 --> ing14_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing14_FeCl3_x_6_H2O -.-> CHEBI_86254
ing15_Na2SO4["Na2SO4"]
CultureMech_009237 --> ing15_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing15_Na2SO4 -.-> CHEBI_32149
ing16_KNO3["KNO3"]
CultureMech_009237 --> ing16_KNO3
CHEBI_63043["CHEBI:63043"]
ing16_KNO3 -.-> CHEBI_63043
ing17_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_009237 --> ing17_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing17_CaSO4_x_2_H2O -.-> CHEBI_32583
ing18_Na2S2O3["Na2S2O3"]
CultureMech_009237 --> ing18_Na2S2O3
CHEBI_32150["CHEBI:32150"]
ing18_Na2S2O3 -.-> CHEBI_32150
ing19_Peptone["Peptone"]
CultureMech_009237 --> ing19_Peptone
ing20_LiCl_Na2WO4_NaSeO3_Ni_NH4_2_SO4___1_mg_ml_each_["LiCl/Na2WO4/NaSeO3/Ni(NH4)2(SO4) (1 mg/ml each)"]
CultureMech_009237 --> ing20_LiCl_Na2WO4_NaSeO3_Ni_NH4_2_SO4___1_mg_ml_each_
ing21_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009237 --> ing21_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing21_Carbon_dioxide_gas -.-> CHEBI_16526
ing22_Nitrogen_gas["Nitrogen gas"]
CultureMech_009237 --> ing22_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing22_Nitrogen_gas -.-> CHEBI_17997
ing23_Distilled_water["Distilled water"]
CultureMech_009237 --> ing23_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing23_Distilled_water -.-> CHEBI_15377
ing24_Na2WO4["Na2WO4"]
CultureMech_009237 --> ing24_Na2WO4
CHEBI_63940["CHEBI:63940"]
ing24_Na2WO4 -.-> CHEBI_63940
ing25_NaSeO3["NaSeO3"]
CultureMech_009237 --> ing25_NaSeO3
ing26_LiCl["LiCl"]
CultureMech_009237 --> ing26_LiCl
CHEBI_48607["CHEBI:48607"]
ing26_LiCl -.-> CHEBI_48607
ing27_Ni_NH4_2_SO4_["Ni(NH4)2(SO4)"]
CultureMech_009237 --> ing27_Ni_NH4_2_SO4_
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Na2MoO4 x2H2O (1 mg/ml) | 30 G_PER_L | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component | |
| 2 | MnCl2 x4H2O (10 mg/ml) | 180 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component | |
| 3 | ZnSO4 x7H2O (10 mg/ml) | 22 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component | |
| 4 | CuCl2 x2H2O (10 mg/ml) | 5 G_PER_L | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound | |
| 5 | CoSO4 x7H2O (1 mg/ml) | 10 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component | |
| 6 | Na2B4O7 x 10 H2O (25 mg/ml) | 180 G_PER_L | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound | |
| 7 | VOSO4 x5H2O (1 mg/ml) | 30 G_PER_L | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound | |
| 8 | Distilled H2O | 1000 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component | |
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 | Yeast Extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 13 | Na2Sx6H2O | 0.13 G_PER_L | Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component | |
| 14 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.02 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.07 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
KNO3
K.NO3 101.102 g/mol
|
0.1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.17 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Na2S2O3
5H2O.2Na.O3S2 248.186 g/mol
|
0.78 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 20 | Peptone | 0.5 G_PER_L | ||
| 21 | LiCl/Na2WO4/NaSeO3/Ni(NH4)2(SO4) (1 mg/ml each) | 10 G_PER_L | ||
| 22 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 23 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 24 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Na2WO4
2Na.O4W 293.816 g/mol
|
1 G_PER_L |
CHEBI:63940
sodium tungstate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 | NaSeO3 | 1 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 27 |
LiCl
Cl.Li 42.394 g/mol
|
1 G_PER_L |
CHEBI:48607
lithium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 | Ni(NH4)2(SO4) | 1 G_PER_L | Role: Mineral source; Properties: Defined component, Simple component |
Solutions
Wolfe's Vitamin Solution (1,000X_x0008_)
Dose: 1 G_PER_L
Role: Growth factor; Properties: Complex component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- obsidian_pool_fermentor_opf_medium_modified_from_m_b_allen_1959 (CultureMech:009237)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.051406Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2682 |
| 2026-01-28T19:48:11.272425Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:18.478308+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:04.564494Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009237 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.309006+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Obsidian Pool fermentor (OPF) medium (modified from M. B. Allen, 1959)" to "obsidian_pool_fermentor_opf_medium_modified_from_m_b_allen_1959" for consistent matching |
| 2026-05-16T09:04:28.049022+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:40.341642+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:28.595564+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:09.711767+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:51.899299+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 521a684c14cedb2ad569bcf335536ca26bf9af24519a8f72d1f765b9aaa564e3 |