pyrolobus_fumarii_medium

CultureMech:009238 archaea COMPLEX LIQUID
Original name: Pyrolobus Fumarii Medium

Overview

External ontology
TOGO:M2683 — Pyrolobus Fumarii Medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M2683 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium792.pdf

Composition

flowchart LR
    CultureMech_009238["pyrolobus_fumarii_medium"]
    ing0_Na2CO3["Na2CO3"]
    CultureMech_009238 --> ing0_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing0_Na2CO3 -.-> CHEBI_29377
    ing1_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009238 --> ing1_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing1_Carbon_dioxide_gas -.-> CHEBI_16526
    ing2_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009238 --> ing2_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing2_Hydrogen_gas -.-> CHEBI_18276
    ing3_Distilled_water["Distilled water"]
    CultureMech_009238 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_009238 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_NaCl["NaCl"]
    CultureMech_009238 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_009238 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_009238 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_H3BO3["H3BO3"]
    CultureMech_009238 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_KCl["KCl"]
    CultureMech_009238 --> ing9_KCl
    CHEBI_32588["CHEBI:32588"]
    ing9_KCl -.-> CHEBI_32588
    ing10_NaBr["NaBr"]
    CultureMech_009238 --> ing10_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing10_NaBr -.-> CHEBI_63004
    ing11_Wolfe_s_mineral_elixir["Wolfe's mineral elixir"]
    CultureMech_009238 --> ing11_Wolfe_s_mineral_elixir
    ing12_KH2PO4["KH2PO4"]
    CultureMech_009238 --> ing12_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing12_KH2PO4 -.-> CHEBI_63036
    ing13_KNO3["KNO3"]
    CultureMech_009238 --> ing13_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing13_KNO3 -.-> CHEBI_63043
    ing14_N2["N2"]
    CultureMech_009238 --> ing14_N2
    CHEBI_17997["CHEBI:17997"]
    ing14_N2 -.-> CHEBI_17997
    ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_009238 --> ing15_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing15_Na2S_x_9_H2O -.-> CHEBI_76209
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_009238 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_009238 --> ing17_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_009238 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_009238 --> ing19_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_009238 --> ing20_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing21_KAl_SO4_2_x_12_H2O["KAl(SO4)2 x 12 H2O"]
    CultureMech_009238 --> ing21_KAl_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing21_KAl_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_009238 --> ing22_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing23_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_009238 --> ing23_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing23_MnSO4_x_H2O -.-> CHEBI_86364
    ing24__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_009238 --> ing24__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing24__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing25_Na2SeO4["Na2SeO4"]
    CultureMech_009238 --> ing25_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing25_Na2SeO4 -.-> CHEBI_77775
    ing26_H2SO4["H2SO4"]
    CultureMech_009238 --> ing26_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing26_H2SO4 -.-> CHEBI_26836
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_009238 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_009238 --> ing28_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing28_Calcium_pantothenate -.-> CHEBI_31345
    ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_009238 --> ing29_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
    CultureMech_009238 --> more["...4 more"]

Ingredients (34)

# Ingredient Concentration Ontology Notes
1 Na2CO3
CO3.2Na 105.988 g/mol
variable VARIABLE CHEBI:29377
sodium carbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Solution
2 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
3 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス
4 Distilled water
H2O 18.015 g/mol
3000.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 980.0, 10.0, 10.0, 1000.0, 1000.0]
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
33.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 3.5, 30.0]
6 NaCl
Cl.Na 58.443 g/mol
23.85 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 13.85, 10.0]
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.38 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.38, 1.0]
8 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.75 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9 H3BO3
H3BO3 61.833 g/mol
15.1 G_PER_L CHEBI:33118
boric acid
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 15.0, 0.1]
10 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 NaBr
Br.Na 102.894 g/mol
0.05 G_PER_L CHEBI:63004
sodium bromide
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12 Wolfe's mineral elixir 1 G_PER_L
13 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
14 KNO3
K.NO3 101.102 g/mol
1 G_PER_L CHEBI:63043
potassium nitrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
15 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
16 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.15 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
18 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
1.8 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
20 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.8 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
21 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22 KAl(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.18 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
23 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
24 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
25 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
2.8 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
26 Na2SeO4
2Na.O4Se 188.936 g/mol
0.1 G_PER_L CHEBI:77775
sodium selenate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
27 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
 Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
28 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
80 G_PER_L CHEBI:30753
4-aminobenzoic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
29 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
100 G_PER_L CHEBI:31345
Calcium pantothenate
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
30 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
300 G_PER_L CHEBI:30961
pyridoxine hydrochloride
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
31 Vitamin B12 100 G_PER_L CHEBI:176843
vitamin B12
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
32 Nicotinic acid
C6H5NO2 123.111 g/mol
200 G_PER_L CHEBI:15940
nicotinic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
33 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
200 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
34 D(+)-Biotin
C10H16N2O3S 244.316 g/mol
20 G_PER_L CHEBI:15956
biotin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component

Solutions

Solution A:

Dose: 989 G_PER_L

Solution B:

Dose: 10 G_PER_L

Solution C:

Dose: 1 G_PER_L

Solution D:

Dose: 10 G_PER_L

Na-resazurin solution (0.1% w/v)

Dose: 0.5 G_PER_L

Role: pH dependent redox indicator; Properties: Solution, Defined component

SrCl2x 6 H2O solution (0.1% w/v)

Dose: 7 G_PER_L

Role: Mineral source; Properties: Solution, Defined component

KI solution (0.01% w/v)

Dose: 0.5 G_PER_L

Role: Mineral source; Properties: Solution, Defined component, Inorganic compound

Seven vitamins solution

Dose: 1 G_PER_L

See mediadive_4611_Seven_vitamins_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.054733Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2683
2026-01-28T19:48:31.292945Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Na2CO3; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2; Added default concentration for ingredient: H2SO4
2026-03-09T08:04:52.135133+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 8 solution(s) from ingredients
2026-03-13T03:09:23.519962Z data-quality-cleanup-v1.0 Data quality fixes Merged 12 duplicate ingredient(s)
2026-03-13T06:20:04.583363Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009238
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:23.327965+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Pyrolobus Fumarii Medium" to "pyrolobus_fumarii_medium" for consistent matching
2026-05-16T09:03:15.198502+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:38.477687+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:33.224464+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 4 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T06:57:28.043727+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:49.633911+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:27.226225+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 153cde0f4b969aea4d5d6306e247963992c86b2cc8445855b166362aa886d636