Overview
- External ontology
- TOGO:M2683 — Pyrolobus Fumarii Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2683 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium792.pdf
Composition
flowchart LR
CultureMech_009238["pyrolobus_fumarii_medium"]
ing0_Na2CO3["Na2CO3"]
CultureMech_009238 --> ing0_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing0_Na2CO3 -.-> CHEBI_29377
ing1_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009238 --> ing1_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing1_Carbon_dioxide_gas -.-> CHEBI_16526
ing2_Hydrogen_gas["Hydrogen gas"]
CultureMech_009238 --> ing2_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing2_Hydrogen_gas -.-> CHEBI_18276
ing3_Distilled_water["Distilled water"]
CultureMech_009238 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009238 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_NaCl["NaCl"]
CultureMech_009238 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009238 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_009238 --> ing7_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
ing8_H3BO3["H3BO3"]
CultureMech_009238 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_KCl["KCl"]
CultureMech_009238 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_NaBr["NaBr"]
CultureMech_009238 --> ing10_NaBr
CHEBI_63004["CHEBI:63004"]
ing10_NaBr -.-> CHEBI_63004
ing11_Wolfe_s_mineral_elixir["Wolfe's mineral elixir"]
CultureMech_009238 --> ing11_Wolfe_s_mineral_elixir
ing12_KH2PO4["KH2PO4"]
CultureMech_009238 --> ing12_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing12_KH2PO4 -.-> CHEBI_63036
ing13_KNO3["KNO3"]
CultureMech_009238 --> ing13_KNO3
CHEBI_63043["CHEBI:63043"]
ing13_KNO3 -.-> CHEBI_63043
ing14_N2["N2"]
CultureMech_009238 --> ing14_N2
CHEBI_17997["CHEBI:17997"]
ing14_N2 -.-> CHEBI_17997
ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_009238 --> ing15_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing15_Na2S_x_9_H2O -.-> CHEBI_76209
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009238 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_009238 --> ing17_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009238 --> ing18_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_009238 --> ing19_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_009238 --> ing20_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
ing21_KAl_SO4_2_x_12_H2O["KAl(SO4)2 x 12 H2O"]
CultureMech_009238 --> ing21_KAl_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing21_KAl_SO4_2_x_12_H2O -.-> CHEBI_86465
ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_009238 --> ing22_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing23_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_009238 --> ing23_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing23_MnSO4_x_H2O -.-> CHEBI_86364
ing24__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_009238 --> ing24__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing24__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing25_Na2SeO4["Na2SeO4"]
CultureMech_009238 --> ing25_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing25_Na2SeO4 -.-> CHEBI_77775
ing26_H2SO4["H2SO4"]
CultureMech_009238 --> ing26_H2SO4
CHEBI_26836["CHEBI:26836"]
ing26_H2SO4 -.-> CHEBI_26836
ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009238 --> ing27_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
ing28_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_009238 --> ing28_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing28_Calcium_pantothenate -.-> CHEBI_31345
ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_009238 --> ing29_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
CultureMech_009238 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2CO3
CO3.2Na 105.988 g/mol
|
variable VARIABLE |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Solution |
| 2 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 3 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 4 |
Distilled water
H2O 18.015 g/mol
|
3000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 980.0, 10.0, 10.0, 1000.0, 1000.0] |
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
33.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 3.5, 30.0] |
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
23.85 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 13.85, 10.0] |
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.38 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.38, 1.0] |
| 8 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.75 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
15.1 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 15.0, 0.1] |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NaBr
Br.Na 102.894 g/mol
|
0.05 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 | Wolfe's mineral elixir | 1 G_PER_L | ||
| 13 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
KNO3
K.NO3 101.102 g/mol
|
1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 16 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.15 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.8 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.8 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
KAl(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.18 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2.8 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.1 G_PER_L |
CHEBI:77775
sodium selenate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 28 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
80 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
100 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
300 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 | Vitamin B12 | 100 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
200 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
200 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
D(+)-Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Solution A:
Dose: 989 G_PER_L
Solution B:
Dose: 10 G_PER_L
Solution C:
Dose: 1 G_PER_L
Solution D:
Dose: 10 G_PER_L
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Solution, Defined component
SrCl2x 6 H2O solution (0.1% w/v)
Dose: 7 G_PER_L
Role: Mineral source; Properties: Solution, Defined component
KI solution (0.01% w/v)
Dose: 0.5 G_PER_L
Role: Mineral source; Properties: Solution, Defined component, Inorganic compound
Seven vitamins solution
Dose: 1 G_PER_L
See mediadive_4611_Seven_vitamins_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.054733Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2683 |
| 2026-01-28T19:48:31.292945Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2CO3; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2; Added default concentration for ingredient: H2SO4 |
| 2026-03-09T08:04:52.135133+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T03:09:23.519962Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 12 duplicate ingredient(s) |
| 2026-03-13T06:20:04.583363Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009238 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.327965+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pyrolobus Fumarii Medium" to "pyrolobus_fumarii_medium" for consistent matching |
| 2026-05-16T09:03:15.198502+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.477687+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:33.224464+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 4 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:28.043727+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.633911+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |