Overview
- External ontology
- TOGO:M2693 — CM4 medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2693 Original URL: https://www.atcc.org/~/media/27E91C31B96E4D15BFD8E349A2115B7F.ashx
Composition
flowchart LR
CultureMech_009245["cm4_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009245 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009245 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_009245 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KH2PO4["KH2PO4"]
CultureMech_009245 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_K2HPO4["K2HPO4"]
CultureMech_009245 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_009245 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_CaCl2["CaCl2"]
CultureMech_009245 --> ing6_CaCl2
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2 -.-> CHEBI_3312
ing7_Carbon_source__i_e__cellobiose_["Carbon source (i.e. cellobiose)"]
CultureMech_009245 --> ing7_Carbon_source__i_e__cellobiose_
ing8_MgCl2["MgCl2"]
CultureMech_009245 --> ing8_MgCl2
CHEBI_6636["CHEBI:6636"]
ing8_MgCl2 -.-> CHEBI_6636
ing9_Agar["Agar"]
CultureMech_009245 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_Sodium_thioglycollate["Sodium thioglycollate"]
CultureMech_009245 --> ing10_Sodium_thioglycollate
CHEBI_86481["CHEBI:86481"]
ing10_Sodium_thioglycollate -.-> CHEBI_86481
ing11_Nitrogen_gas["Nitrogen gas"]
CultureMech_009245 --> ing11_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing11_Nitrogen_gas -.-> CHEBI_17997
ing12_Hydrogen_gas["Hydrogen gas"]
CultureMech_009245 --> ing12_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing12_Hydrogen_gas -.-> CHEBI_18276
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
CaCl2
Ca.2Cl 110.984 g/mol
|
13.2 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 | Carbon source (i.e. cellobiose) | 6 G_PER_L | Role: Carbon source; Properties: Simple component, Defined component, Organic compound | |
| 9 |
MgCl2
2Cl.Mg 95.211 g/mol
|
0.75 G_PER_L |
CHEBI:6636
magnesium dichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 10 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 11 |
Sodium thioglycollate
C2H3O2S.Na 114.101 g/mol
|
0.5 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Properties: Defined component, Organic compound, Simple component |
| 12 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 13 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Solutions
FeSO4 (1.25% solution)
Dose: 0.1 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Solution, Defined component
Resazurin (1.0% solution)
Dose: 0.2 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- cm4_medium (CultureMech:009245)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.068480Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2693 |
| 2026-01-28T19:48:10.176993Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:16.615279+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:04.640170Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009245 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.388430+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CM4 medium" to "cm4_medium" for consistent matching |
| 2026-05-16T09:03:28.077462+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:54.519745+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.921608+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:28.880270+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2686af5f1756524ab263e4b4babb0ae2292c46406a018d4d6fa0108744e36e4c |