cm4_medium (CultureMech:009245)

Overview

External ontology

TOGO:M2693 — CM4 medium

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M2693 Original URL: https://www.atcc.org/~/media/27E91C31B96E4D15BFD8E349A2115B7F.ashx

Composition

flowchart LR
    CultureMech_009245["cm4_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009245 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_009245 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_009245 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009245 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_K2HPO4["K2HPO4"]
    CultureMech_009245 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009245 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_CaCl2["CaCl2"]
    CultureMech_009245 --> ing6_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2 -.-> CHEBI_3312
    ing7_Carbon_source__i_e__cellobiose_["Carbon source (i.e. cellobiose)"]
    CultureMech_009245 --> ing7_Carbon_source__i_e__cellobiose_
    ing8_MgCl2["MgCl2"]
    CultureMech_009245 --> ing8_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing8_MgCl2 -.-> CHEBI_6636
    ing9_Agar["Agar"]
    CultureMech_009245 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509
    ing10_Sodium_thioglycollate["Sodium thioglycollate"]
    CultureMech_009245 --> ing10_Sodium_thioglycollate
    CHEBI_86481["CHEBI:86481"]
    ing10_Sodium_thioglycollate -.-> CHEBI_86481
    ing11_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009245 --> ing11_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing11_Nitrogen_gas -.-> CHEBI_17997
    ing12_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009245 --> ing12_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing12_Hydrogen_gas -.-> CHEBI_18276

Ingredients (13)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2	Yeast extract	5 G_PER_L		Role: Nutrient source; Properties: Undefined component
3	NaCl Cl.Na 58.443 g/mol	1 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4	KH2PO4 H2O4P.K 136.084 g/mol	1.5 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5	K2HPO4 HO4P.2K 174.174 g/mol	2.9 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
6	(NH4)2SO4 2H4N.O4S 132.141 g/mol	1.3 G_PER_L	CHEBI:62946 ammonium sulfate	Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
7	CaCl2 Ca.2Cl 110.984 g/mol	13.2 G_PER_L	CHEBI:3312 calcium dichloride	Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
8	Carbon source (i.e. cellobiose)	6 G_PER_L		Role: Carbon source; Properties: Simple component, Defined component, Organic compound
9	MgCl2 2Cl.Mg 95.211 g/mol	0.75 G_PER_L	CHEBI:6636 magnesium dichloride	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10	Agar	20 G_PER_L	CHEBI:2509 agar	Role: Solidifying component
11	Sodium thioglycollate C2H3O2S.Na 114.101 g/mol	0.5 G_PER_L	CHEBI:86481 sodium thioglycolate	Properties: Defined component, Organic compound, Simple component
12	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス
13	Hydrogen gas H2 2.016 g/mol	variable VARIABLE	CHEBI:18276 dihydrogen	Properties: ガス

Solutions

FeSO4 (1.25% solution)

Dose: 0.1 G_PER_L

Role: Mineral source; Properties: Inorganic compound, Solution, Defined component

Resazurin (1.0% solution)

Dose: 0.2 G_PER_L

Role: pH dependent redox indicator; Properties: Defined component, Solution

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:57.068480Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2693
2026-01-28T19:48:10.176993Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:16.615279+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 2 solution(s) from ingredients
2026-03-13T06:20:04.640170Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009245
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name
2026-03-17T06:29:46.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:23.388430+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "CM4 medium" to "cm4_medium" for consistent matching
2026-05-16T09:03:28.077462+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:54.519745+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.921608+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.