Overview
- External ontology
- TOGO:M269 — Castenholz Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M269 Original source: JCM - JCM_M276 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=276
Composition
flowchart LR
CultureMech_009252["castenholz_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009252 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009252 --> ing1_Yeast_extract__BD_Difco_
ing2_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_009252 --> ing2_Tryptone__BD_Difco_
ing3_NaOH["NaOH"]
CultureMech_009252 --> ing3_NaOH
CHEBI_32145["CHEBI:32145"]
ing3_NaOH -.-> CHEBI_32145
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
900 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 3 | Tryptone (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 4 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Solutions
Castenholz basal salt solution (see Medium [M266])
Dose: 100 G_PER_L
Cross-reference to Medium M266
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M269_Castenholz_Medium (CultureMech:009252)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.735777Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M269 |
| 2026-01-28T19:48:12.393734Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:20.457847+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:04.706093Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009252 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.457849+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Castenholz Medium" to "castenholz_medium" for consistent matching |
| 2026-05-16T09:03:15.258725+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.541249+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:13.056760+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8c82a56188161983b2eab7b6464340bcce84e1d7ea9e2b50aab5f4ed23abe484 |