Overview
- External ontology
- TOGO:M269 — Castenholz Medium
- Applications
-
- Microbial cultivation
- pH
- 8.2
- Notes
- TOGO M269 imports JCM Medium 276 Castenholz Medium. JCM Medium 276 lists 1.0 g Tryptone, 1.0 g Yeast extract, 100.0 ml Castenholz basal salt solution from JCM Medium 273, and 900.0 ml Distilled water, then adjusts pH to 8.2 with NaOH.
Composition
flowchart LR
CultureMech_009252["castenholz_medium"]
ing0_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_009252 --> ing0_Tryptone__BD_Difco_
ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009252 --> ing1_Yeast_extract__BD_Difco_
ing2_Distilled_water["Distilled water"]
CultureMech_009252 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone (BD-Difco) | 1.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
JCM Medium 276 lists 1.0 g/L Tryptone from BD-Difco. |
| 2 | Yeast extract (BD-Difco) | 1.0 G_PER_L |
FOODON:03315426
yeast extract
|
JCM Medium 276 lists 1.0 g/L Yeast extract from BD-Difco. |
| 3 |
Distilled water
H2O 18.015 g/mol
|
900.0 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 276 lists 900.0 ml/L Distilled water. |
Preparation
- Adjust pH to 8.2 with NaOH.
- Autoclave at 121 degrees C for 15 minutes.
Solutions
Castenholz basal salt solution (see Medium No. 273)
Dose: 100.0 ML_PER_L
JCM Medium 276 adds 100.0 ml/L Castenholz basal salt solution from JCM Medium 273.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.735777Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M269 |
| 2026-01-28T19:48:12.393734Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:20.457847+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:04.706093Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009252 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.457849+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Castenholz Medium" to "castenholz_medium" for consistent matching |
| 2026-05-16T09:03:15.258725+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.541249+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m269_castenholz_score15.py | RESOLVED_TOGO_M269_CASTENHOLZ_SCORE15 | TOGO M269 imports JCM Medium 276 Castenholz Medium. JCM Medium 276 lists 1.0 g Tryptone, 1.0 g Yeast extract, 100.0 ml Castenholz basal salt solution from JCM Medium 273, and 900.0 ml Distilled water, then adjusts pH to 8.2 with NaOH. Corrected the imported water and basal-salt solution unit artifacts, removed NaOH from the ingredient list because it is only the pH titrant, grounded yeast extract and tryptone, and linked the record to its existing JCM Medium 276 source duplicate. |