Overview
- Notes
- MediaDive solution 3963 is the Castenholz basal salt solution from JCM Medium 273 used by JCM Medium 276 at 100.0 ml/L.
Stock composition (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 ML_PER_L |
CHEBI:15377
water
|
MediaDive solution 3963 / JCM Medium 273 lists 1000.0 ml/L Distilled water. |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
MediaDive solution 3963 / JCM Medium 273 lists 1.0 g/L MgSO4 x 7 H2O. |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.08 G_PER_L |
CHEBI:26710
sodium chloride
|
MediaDive solution 3963 / JCM Medium 273 lists 0.08 g/L NaCl. |
| 4 |
NaNO3
NO3.Na 84.994 g/mol
|
6.89 G_PER_L |
CHEBI:63005
sodium nitrate
|
MediaDive solution 3963 / JCM Medium 273 lists 6.89 g/L NaNO3. |
| 5 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.0 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
MediaDive solution 3963 / JCM Medium 273 lists 1.0 g/L Nitrilotriacetic acid. |
| 6 |
KNO3
K.NO3 101.102 g/mol
|
1.03 G_PER_L |
CHEBI:63043
potassium nitrate
|
MediaDive solution 3963 / JCM Medium 273 lists 1.03 g/L KNO3. |
| 7 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
1.11 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
MediaDive solution 3963 / JCM Medium 273 lists 1.11 g/L Na2HPO4. |
| 8 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.6 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
MediaDive solution 3963 / JCM Medium 273 lists 0.6 g/L CaSO4 x 2 H2O. |
Solutions
FeCl3 x 6 H2O solution (0.03%)
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeCl3 x 6 H2O | 0.03 PERCENT_W_V | MediaDive solution 3963 / JCM Medium 273 lists 0.03 % w/v FeCl3 x 6 H2O. |
MediaDive solution 3963 / JCM Medium 273 adds 10.0 ml/L FeCl3 x 6 H2O solution at 0.03% w/v.
Nitsch's trace elements
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1000.0 ML_PER_L | MediaDive solution 3963 / JCM Medium 273 lists 1000.0 ml/L Distilled water. |
| Na2MoO4 x 2 H2O | 0.025 G_PER_L | MediaDive solution 3963 / JCM Medium 273 lists 0.025 g/L Na2MoO4 x 2 H2O. |
| H3BO3 | 0.5 G_PER_L | MediaDive solution 3963 / JCM Medium 273 lists 0.5 g/L H3BO3. |
| CoCl2 x 6 H2O | 0.046 G_PER_L | MediaDive solution 3963 / JCM Medium 273 lists 0.046 g/L CoCl2 x 6 H2O. |
| ZnSO4 x 7 H2O | 0.5 G_PER_L | MediaDive solution 3963 / JCM Medium 273 lists 0.5 g/L ZnSO4 x 7 H2O. |
| CuSO4 x 5 H2O | 0.016 G_PER_L | MediaDive solution 3963 / JCM Medium 273 lists 0.016 g/L CuSO4 x 5 H2O. |
| MnSO4 x n H2O | 2.2 G_PER_L | MediaDive solution 3963 / JCM Medium 273 lists 2.2 g/L MnSO4 x n H2O. |
| H2SO4 | 0.5 ML_PER_L | MediaDive solution 3963 / JCM Medium 273 lists 0.5 ml/L H2SO4. |
MediaDive solution 3963 / JCM Medium 273 adds 10.0 ml/L Nitsch's trace elements.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-03-13T06:20:28.949424Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:013022 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-03-14T21:09:33.544606 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-04-04T17:50:20.357310 | categorize_unknown_media | CATEGORIZED | Automated categorization (confidence: 0.30). No clear match, defaulting to bacterial |
| 2026-05-16T09:03:56.489441+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-10T06:38:29.605989+00:00 | kg-fallback-consensus-v1.0 | Replaced low-confidence kg_fallback chebi_term with corpus consensus | Re-grounded 3 chebi_term(s) to the unanimous reliable CHEBI for the ingredient label seen elsewhere in the corpus (G25 Phase 1, zero external lookups). |
| 2026-06-10T06:48:14.313902+00:00 | kg-fallback-oak-ols-v1.0 | Resolved/de-grounded low-confidence chebi_term via OAK+OLS (G25 Phase 2) | 2 chebi_term(s): OAK sqlite:obo:chebi + OLS4 exact agreement, corpus-majority disambiguation, or mixture de-ground. |
| 2026-06-10T06:56:17.301545+00:00 | kg-fallback-llm-curation-v1.0 | LLM-curated low-confidence chebi_term (G25 Phase 3) | 1 chebi_term(s): curator-named compound resolved via OAK+OLS, or de-grounded as a mixture, or confirmed correct. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m935_microaerophilic_thermus_score15.py | RESOLVED_MEDIADIVE_3963_CASTENHOLZ_BASAL_SALT | Restored the JCM Medium 273 Castenholz stock concentrations, corrected false percent-volume FeCl3 and water rows to the source FeCl3 stock addition plus 1000.0 ml/L water, restored the missing Nitsch's trace-elements addition, and removed the placeholder top-level ingredient. |