Nitsch's trace elements

CultureMech:013023 bacterial

Overview

Notes
MediaDive solution 3964 is the Nitsch's trace-elements stock used by Castenholz basal salt solution at 10.0 ml/L.

Stock composition (8)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1000.0 ML_PER_L CHEBI:15377
water
MediaDive solution 3964 / JCM Medium 273 lists 1000.0 ml/L Distilled water.
2 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.025 G_PER_L CHEBI:75213
sodium molybdate dihydrate
MediaDive solution 3964 / JCM Medium 273 lists 0.025 g/L Na2MoO4 x 2 H2O.
3 H3BO3
H3BO3 61.833 g/mol
0.5 G_PER_L CHEBI:33118
boric acid
MediaDive solution 3964 / JCM Medium 273 lists 0.5 g/L H3BO3.
4 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.046 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
MediaDive solution 3964 / JCM Medium 273 lists 0.046 g/L CoCl2 x 6 H2O.
5 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.5 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
MediaDive solution 3964 / JCM Medium 273 lists 0.5 g/L ZnSO4 x 7 H2O.
6 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.016 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
MediaDive solution 3964 / JCM Medium 273 lists 0.016 g/L CuSO4 x 5 H2O.
7 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
2.2 G_PER_L CHEBI:86360
manganese(II) sulfate
MediaDive solution 3964 / JCM Medium 273 lists 2.2 g/L MnSO4 x n H2O.
8 H2SO4
H2O4S 98.079 g/mol
0.5 ML_PER_L CHEBI:26836
sulfuric acid
MediaDive solution 3964 / JCM Medium 273 lists 0.5 ml/L H2SO4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-03-13T06:20:28.955511Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:013023
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-03-14T21:09:33.550418 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-04-04T17:49:56.316722 categorize_unknown_media CATEGORIZED Automated categorization (confidence: 0.30). No clear match, defaulting to bacterial
2026-05-16T09:03:56.496181+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-10T06:38:29.612558+00:00 kg-fallback-consensus-v1.0 Replaced low-confidence kg_fallback chebi_term with corpus consensus Re-grounded 5 chebi_term(s) to the unanimous reliable CHEBI for the ingredient label seen elsewhere in the corpus (G25 Phase 1, zero external lookups).
2026-06-10T06:48:14.320975+00:00 kg-fallback-oak-ols-v1.0 Resolved/de-grounded low-confidence chebi_term via OAK+OLS (G25 Phase 2) 1 chebi_term(s): OAK sqlite:obo:chebi + OLS4 exact agreement, corpus-majority disambiguation, or mixture de-ground.
2026-09-11T00:00:00-07:00 repair_togo_m935_microaerophilic_thermus_score15.py RESOLVED_MEDIADIVE_3964_NITSCH_TRACE_ELEMENTS Corrected the Nitsch stock water and H2SO4 rows from false percent-volume units to the source 1000.0 ml/L and 0.5 ml/L amounts, replaced upstream mediadive.compound groundings with direct ontology mappings, and removed the placeholder top-level ingredient.