cmc_medium_n2_co2

CultureMech:009268 bacterial COMPLEX LIQUID
Original name: CMC Medium (N2/CO2)

Overview

External ontology
TOGO:M2717 — CMC Medium (N2/CO2)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2717 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium110a.pdf

Composition

flowchart LR
    CultureMech_009268["cmc_medium_n2_co2"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_009268 --> ing0_Yeast_extract
    ing1_K2HPO4["K2HPO4"]
    CultureMech_009268 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_D_Glucose["D-Glucose"]
    CultureMech_009268 --> ing2_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing2_D_Glucose -.-> CHEBI_17634
    ing3_Na2CO3["Na2CO3"]
    CultureMech_009268 --> ing3_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing3_Na2CO3 -.-> CHEBI_29377
    ing4_Cellobiose["Cellobiose"]
    CultureMech_009268 --> ing4_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing4_Cellobiose -.-> CHEBI_17057
    ing5_Maltose["Maltose"]
    CultureMech_009268 --> ing5_Maltose
    CHEBI_18167["CHEBI:18167"]
    ing5_Maltose -.-> CHEBI_18167
    ing6_Starch__soluble_["Starch (soluble)"]
    CultureMech_009268 --> ing6_Starch__soluble_
    ing7_Casitone["Casitone"]
    CultureMech_009268 --> ing7_Casitone
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine-HCl x H2O"]
    CultureMech_009268 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Meat_filtrate["Meat filtrate"]
    CultureMech_009268 --> ing9_Meat_filtrate
    ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009268 --> ing10_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing10_Carbon_dioxide_gas -.-> CHEBI_16526
    ing11_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009268 --> ing11_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing11_Nitrogen_gas -.-> CHEBI_17997
    ing12_Distilled_water["Distilled water"]
    CultureMech_009268 --> ing12_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing12_Distilled_water -.-> CHEBI_15377
    ing13_NaOH_1_N["NaOH 1 N"]
    CultureMech_009268 --> ing13_NaOH_1_N
    ing14_Ground_beef__fat_free_["Ground beef (fat free)"]
    CultureMech_009268 --> ing14_Ground_beef__fat_free_

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
2 K2HPO4
HO4P.2K 174.174 g/mol
5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
3 D-Glucose
C6H12O6 180.156 g/mol
4 G_PER_L CHEBI:17634
D-glucose
 Role: Carbon source; Properties: Simple component, Organic compound, Defined component
4 Na2CO3
CO3.2Na 105.988 g/mol
1.5 G_PER_L CHEBI:29377
sodium carbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
5 Cellobiose
C12H22O11 342.297 g/mol
1 G_PER_L CHEBI:17057
cellobiose
 Role: Carbon source; Properties: Organic compound, Defined component, Simple component
6 Maltose
C12H22O11 342.297 g/mol
1 G_PER_L CHEBI:18167
alpha-maltose
 Role: Carbon source; Properties: Defined component, Organic compound, Simple component
7 Starch (soluble) 1 G_PER_L Role: Carbon source; Properties: Undefined component
8 Casitone 30 G_PER_L Properties: Undefined component
9 L-Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Organic compound, Simple component, Defined component
10 Meat filtrate 1000 G_PER_L
11 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
12 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
13 Distilled water
H2O 18.015 g/mol
1000 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
14 NaOH 1 N 25 G_PER_L Properties: Defined component, Solution, Inorganic compound
15 Ground beef (fat free) 500 G_PER_L Properties: Undefined component

Solutions

Na-resazurin solution (0.1% w/v)

Dose: 0.5 G_PER_L

Role: pH dependent redox indicator; Properties: Defined component, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.123023Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2717
2026-01-28T19:48:29.066868Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:48.196054+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:04.890063Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009268
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:23.654345+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CMC Medium (N2/CO2)" to "cmc_medium_n2_co2" for consistent matching
2026-05-16T09:03:15.390060+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:38.672388+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:49.706019+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:13.226941+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint a5cfe0a50c606f7e0c689740836a05220840857a5c1bdafcf6589d80d53dea32