Overview
- External ontology
- TOGO:M2717 — CMC Medium (N2/CO2)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2717 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium110a.pdf
Composition
flowchart LR
CultureMech_009268["cmc_medium_n2_co2"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_009268 --> ing0_Yeast_extract
ing1_K2HPO4["K2HPO4"]
CultureMech_009268 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_D_Glucose["D-Glucose"]
CultureMech_009268 --> ing2_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing2_D_Glucose -.-> CHEBI_17634
ing3_Na2CO3["Na2CO3"]
CultureMech_009268 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_Cellobiose["Cellobiose"]
CultureMech_009268 --> ing4_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing4_Cellobiose -.-> CHEBI_17057
ing5_Maltose["Maltose"]
CultureMech_009268 --> ing5_Maltose
CHEBI_18167["CHEBI:18167"]
ing5_Maltose -.-> CHEBI_18167
ing6_Starch__soluble_["Starch (soluble)"]
CultureMech_009268 --> ing6_Starch__soluble_
ing7_Casitone["Casitone"]
CultureMech_009268 --> ing7_Casitone
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine-HCl x H2O"]
CultureMech_009268 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Meat_filtrate["Meat filtrate"]
CultureMech_009268 --> ing9_Meat_filtrate
ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009268 --> ing10_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing10_Carbon_dioxide_gas -.-> CHEBI_16526
ing11_Nitrogen_gas["Nitrogen gas"]
CultureMech_009268 --> ing11_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing11_Nitrogen_gas -.-> CHEBI_17997
ing12_Distilled_water["Distilled water"]
CultureMech_009268 --> ing12_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing12_Distilled_water -.-> CHEBI_15377
ing13_NaOH_1_N["NaOH 1 N"]
CultureMech_009268 --> ing13_NaOH_1_N
ing14_Ground_beef__fat_free_["Ground beef (fat free)"]
CultureMech_009268 --> ing14_Ground_beef__fat_free_
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
D-Glucose
C6H12O6 180.156 g/mol
|
4 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Cellobiose
C12H22O11 342.297 g/mol
|
1 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 |
Maltose
C12H22O11 342.297 g/mol
|
1 G_PER_L |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 7 | Starch (soluble) | 1 G_PER_L | Role: Carbon source; Properties: Undefined component | |
| 8 | Casitone | 30 G_PER_L | Properties: Undefined component | |
| 9 |
L-Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Organic compound, Simple component, Defined component |
| 10 | Meat filtrate | 1000 G_PER_L | ||
| 11 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 12 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 13 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 14 | NaOH 1 N | 25 G_PER_L | Properties: Defined component, Solution, Inorganic compound | |
| 15 | Ground beef (fat free) | 500 G_PER_L | Properties: Undefined component |
Solutions
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.123023Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2717 |
| 2026-01-28T19:48:29.066868Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:48.196054+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:04.890063Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009268 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.654345+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CMC Medium (N2/CO2)" to "cmc_medium_n2_co2" for consistent matching |
| 2026-05-16T09:03:15.390060+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.672388+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.706019+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |