4_yaco_media

CultureMech:009318 bacterial COMPLEX LIQUID
Original name: 4-YACo media

Overview

External ontology
TOGO:M2770 — 4-YACo media
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2770

Composition

flowchart LR
    CultureMech_009318["4_yaco_media"]
    ing0_yeast_autolysate["yeast autolysate"]
    CultureMech_009318 --> ing0_yeast_autolysate
    ing1_maltose["maltose"]
    CultureMech_009318 --> ing1_maltose
    CHEBI_18167["CHEBI:18167"]
    ing1_maltose -.-> CHEBI_18167
    ing2_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009318 --> ing2_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing2_Carbon_dioxide_gas -.-> CHEBI_16526
    ing3_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009318 --> ing3_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing3_Hydrogen_gas -.-> CHEBI_18276
    ing4_Vitamin_B7["Vitamin B7"]
    CultureMech_009318 --> ing4_Vitamin_B7
    CHEBI_15956["CHEBI:15956"]
    ing4_Vitamin_B7 -.-> CHEBI_15956
    ing5_Vitamin_B6["Vitamin B6"]
    CultureMech_009318 --> ing5_Vitamin_B6
    CHEBI_16709["CHEBI:16709"]
    ing5_Vitamin_B6 -.-> CHEBI_16709
    ing6_Vitamin_B12["Vitamin B12"]
    CultureMech_009318 --> ing6_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing6_Vitamin_B12 -.-> CHEBI_176843
    ing7_Tryptophan["Tryptophan"]
    CultureMech_009318 --> ing7_Tryptophan
    CHEBI_27897["CHEBI:27897"]
    ing7_Tryptophan -.-> CHEBI_27897
    ing8_biotin__Sigma_Aldrich__St_Louis__MO__USA_["biotin (Sigma Aldrich, St Louis, MO, USA)"]
    CultureMech_009318 --> ing8_biotin__Sigma_Aldrich__St_Louis__MO__USA_
    ing9_pyridoxal_phosphate__Sigma_Aldrich_["pyridoxal-phosphate (Sigma Aldrich)"]
    CultureMech_009318 --> ing9_pyridoxal_phosphate__Sigma_Aldrich_
    ing10_pyridoxal_HCl__Sigma_Aldrich_["pyridoxal-HCl (Sigma Aldrich)"]
    CultureMech_009318 --> ing10_pyridoxal_HCl__Sigma_Aldrich_
    ing11_hydroxocobalamin_acetate_salt["hydroxocobalamin acetate salt"]
    CultureMech_009318 --> ing11_hydroxocobalamin_acetate_salt
    ing12_hydroxocobalamin_hydrochloride["hydroxocobalamin hydrochloride"]
    CultureMech_009318 --> ing12_hydroxocobalamin_hydrochloride
    ing13_methylcobalamin["methylcobalamin"]
    CultureMech_009318 --> ing13_methylcobalamin
    ing14_cyanocobalamin__Sigma_Aldrich_["cyanocobalamin (Sigma Aldrich)"]
    CultureMech_009318 --> ing14_cyanocobalamin__Sigma_Aldrich_
    ing15_tryptophan__Sigma_Aldrich_["tryptophan (Sigma Aldrich)"]
    CultureMech_009318 --> ing15_tryptophan__Sigma_Aldrich_

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 yeast autolysate 4 PERCENT_W_V Properties: Undefined component
2 maltose
C12H22O11 342.297 g/mol
variable VARIABLE CHEBI:18167
alpha-maltose
Role: Carbon source; Properties: Organic compound, Simple component, Defined component
3 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
Properties: ガス
4 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
Properties: ガス
5 Vitamin B7
C10H16N2O3S 244.316 g/mol
variable VARIABLE CHEBI:15956
biotin
6 Vitamin B6
C8H11NO3 169.18 g/mol
variable VARIABLE CHEBI:16709
pyridoxine
7 Vitamin B12 variable VARIABLE CHEBI:176843
vitamin B12
8 Tryptophan
C11H12N2O2 204.229 g/mol
variable VARIABLE CHEBI:27897
tryptophan
9 biotin (Sigma Aldrich, St Louis, MO, USA) variable VARIABLE Role: Vitamin; Properties: Defined component, Organic compound, Simple component
10 pyridoxal-phosphate (Sigma Aldrich) variable VARIABLE Role: Vitamin; Properties: Organic compound, Simple component, Defined component
11 pyridoxal-HCl (Sigma Aldrich) variable VARIABLE Role: Vitamin
12 hydroxocobalamin acetate salt variable VARIABLE Role: Growth factor; Properties: Organic compound, Simple component, Defined component
13 hydroxocobalamin hydrochloride variable VARIABLE Role: Growth factor; Properties: Simple component, Defined component
14 methylcobalamin variable VARIABLE Role: Growth factor
15 cyanocobalamin (Sigma Aldrich) variable VARIABLE Role: Vitamin
16 tryptophan (Sigma Aldrich) variable VARIABLE Role: Nitrogen source; Properties: Simple component, Organic compound, Defined component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.273356Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2770
2026-01-28T19:48:37.003151Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: maltose; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Vitamin B7; Added default concentration for ingredient: Vitamin B6; Added default concentration for ingredient: Vitamin B12; Added default concentration for ingredient: Tryptophan; Added default concentration for ingredient: biotin (Sigma Aldrich, St Louis, MO, USA); Added default concentration for ingredient: pyridoxal-phosphate (Sigma Aldrich); Added default concentration for ingredient: pyridoxal-HCl (Sigma Aldrich); Added default concentration for ingredient: hydroxocobalamin acetate salt; Added default concentration for ingredient: hydroxocobalamin hydrochloride; Added default concentration for ingredient: methylcobalamin; Added default concentration for ingredient: cyanocobalamin (Sigma Aldrich); Added default concentration for ingredient: tryptophan (Sigma Aldrich)
2026-03-13T06:20:05.596711Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009318
2026-03-17T06:30:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.505281+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "4-YACo media" to "4_yaco_media" for consistent matching
2026-05-16T09:02:58.297779+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:15.845866+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:04:34.128575+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 52fa62fcd8f2c7bdbc4cd89c821d0fbc5da3ca94c67bbfb83bf406eff887d733