modified_mrs

CultureMech:009331 bacterial COMPLEX LIQUID
Original name: Modified-MRS

Overview

External ontology
TOGO:M2782 — Modified-MRS
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2782

Composition

flowchart LR
    CultureMech_009331["modified_mrs"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009331 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4.7H2O"]
    CultureMech_009331 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_009331 --> ing2_Yeast_extract
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009331 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_K2HPO4["K2HPO4"]
    CultureMech_009331 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_FeSO4_7H2O["FeSO4.7H2O"]
    CultureMech_009331 --> ing5_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_7H2O -.-> CHEBI_75836
    ing6_Polysorbate_80["Polysorbate 80"]
    CultureMech_009331 --> ing6_Polysorbate_80
    CHEBI_53426["CHEBI:53426"]
    ing6_Polysorbate_80 -.-> CHEBI_53426
    ing7_MnSO4_4H2O["MnSO4.4H2O"]
    CultureMech_009331 --> ing7_MnSO4_4H2O
    CHEBI_86358["CHEBI:86358"]
    ing7_MnSO4_4H2O -.-> CHEBI_86358
    ing8_Tri_ammonium_citrate["Tri-ammonium citrate"]
    CultureMech_009331 --> ing8_Tri_ammonium_citrate
    ing9_Pyruvic_acid["Pyruvic acid"]
    CultureMech_009331 --> ing9_Pyruvic_acid
    CHEBI_32816["CHEBI:32816"]
    ing9_Pyruvic_acid -.-> CHEBI_32816
    ing10_Cysteine_HCl["Cysteine-HCl"]
    CultureMech_009331 --> ing10_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing10_Cysteine_HCl -.-> CHEBI_91247
    ing11_Meat_extract["Meat extract"]
    CultureMech_009331 --> ing11_Meat_extract
    ing12_Peptone_from_casein["Peptone from casein"]
    CultureMech_009331 --> ing12_Peptone_from_casein
    ing13_Maltose["Maltose"]
    CultureMech_009331 --> ing13_Maltose
    CHEBI_18167["CHEBI:18167"]
    ing13_Maltose -.-> CHEBI_18167

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
0.575 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
4 KH2PO4
H2O4P.K 136.084 g/mol
3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
5 K2HPO4
HO4P.2K 174.174 g/mol
3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
6 FeSO4.7H2O
Fe.7H2O.O4S 278.013 g/mol
0.034 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
7 Polysorbate 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1 G_PER_L CHEBI:53426
polysorbate 80
 Role: Surfactant; Properties: Organic compound, Simple component, Defined component
8 MnSO4.4H2O
4H2O.Mn.O4S 223.061 g/mol
0.12 G_PER_L CHEBI:86358
manganese(II) sulfate tetrahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
9 Tri-ammonium citrate 2 G_PER_L Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component
10 Pyruvic acid
C3H4O3 88.062 g/mol
0.2 G_PER_L CHEBI:32816
pyruvic acid
 Role: Carbon source
11 Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
0.3 G_PER_L CHEBI:91247
L-cysteine hydrochloride
 Properties: Organic compound, Simple component, Defined component [WARNING: 2 duplicates with conflicting units] [MANUAL REVIEW REQUIRED: PERCENT_W_V incompatible with absolute units]
12 Meat extract 10 G_PER_L Properties: Undefined component
13 Peptone from casein 10 G_PER_L Properties: Undefined component
14 Maltose
C12H22O11 342.297 g/mol
1 PERCENT_W_V CHEBI:18167
alpha-maltose
 Role: Carbon source; Properties: Defined component, Organic compound, Simple component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.295193Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2782
2026-01-28T19:48:36.817233Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:33.294105Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s); WARNING: Duplicate 'Cysteine-HCl' with different units: {'G_PER_L', 'PERCENT_W_V'}
2026-03-13T04:23:43.789626Z unit-conflict-resolver-v1.0 Resolved unit conflicts Flagged Cysteine-HCl for manual review (PERCENT_W_V conflict)
2026-03-13T06:20:05.694918Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009331
2026-03-17T06:30:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.609706+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified-MRS" to "modified_mrs" for consistent matching
2026-06-05T07:41:37.443554+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.853773+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:49.196578+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 72e4601be8283759e39b4aa85b205e6465f4d2c49ee2906c4c94f474d41b2721