Overview
- External ontology
- TOGO:M2782 — Modified-MRS
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2782
Composition
flowchart LR
CultureMech_009331["modified_mrs"]
ing0_Distilled_water["Distilled water"]
CultureMech_009331 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4.7H2O"]
CultureMech_009331 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009331 --> ing2_Yeast_extract
ing3_KH2PO4["KH2PO4"]
CultureMech_009331 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_K2HPO4["K2HPO4"]
CultureMech_009331 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_FeSO4_7H2O["FeSO4.7H2O"]
CultureMech_009331 --> ing5_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_7H2O -.-> CHEBI_75836
ing6_Polysorbate_80["Polysorbate 80"]
CultureMech_009331 --> ing6_Polysorbate_80
CHEBI_53426["CHEBI:53426"]
ing6_Polysorbate_80 -.-> CHEBI_53426
ing7_MnSO4_4H2O["MnSO4.4H2O"]
CultureMech_009331 --> ing7_MnSO4_4H2O
CHEBI_86358["CHEBI:86358"]
ing7_MnSO4_4H2O -.-> CHEBI_86358
ing8_Tri_ammonium_citrate["Tri-ammonium citrate"]
CultureMech_009331 --> ing8_Tri_ammonium_citrate
ing9_Pyruvic_acid["Pyruvic acid"]
CultureMech_009331 --> ing9_Pyruvic_acid
CHEBI_32816["CHEBI:32816"]
ing9_Pyruvic_acid -.-> CHEBI_32816
ing10_Cysteine_HCl["Cysteine-HCl"]
CultureMech_009331 --> ing10_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing10_Cysteine_HCl -.-> CHEBI_91247
ing11_Meat_extract["Meat extract"]
CultureMech_009331 --> ing11_Meat_extract
ing12_Peptone_from_casein["Peptone from casein"]
CultureMech_009331 --> ing12_Peptone_from_casein
ing13_Maltose["Maltose"]
CultureMech_009331 --> ing13_Maltose
CHEBI_18167["CHEBI:18167"]
ing13_Maltose -.-> CHEBI_18167
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.575 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
FeSO4.7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.034 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
Polysorbate 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Organic compound, Simple component, Defined component |
| 8 |
MnSO4.4H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.12 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 9 | Tri-ammonium citrate | 2 G_PER_L | Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component | |
| 10 |
Pyruvic acid
C3H4O3 88.062 g/mol
|
0.2 G_PER_L |
CHEBI:32816
pyruvic acid
|
Role: Carbon source |
| 11 |
Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.3 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
Properties: Organic compound, Simple component, Defined component [WARNING: 2 duplicates with conflicting units] [MANUAL REVIEW REQUIRED: PERCENT_W_V incompatible with absolute units] |
| 12 | Meat extract | 10 G_PER_L | Properties: Undefined component | |
| 13 | Peptone from casein | 10 G_PER_L | Properties: Undefined component | |
| 14 |
Maltose
C12H22O11 342.297 g/mol
|
1 PERCENT_W_V |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.295193Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2782 |
| 2026-01-28T19:48:36.817233Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:33.294105Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s); WARNING: Duplicate 'Cysteine-HCl' with different units: {'G_PER_L', 'PERCENT_W_V'} |
| 2026-03-13T04:23:43.789626Z | unit-conflict-resolver-v1.0 | Resolved unit conflicts | Flagged Cysteine-HCl for manual review (PERCENT_W_V conflict) |
| 2026-03-13T06:20:05.694918Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009331 |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.609706+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified-MRS" to "modified_mrs" for consistent matching |
| 2026-06-05T07:41:37.443554+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.853773+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |