nutrient_broth

CultureMech:009332 bacterial COMPLEX LIQUID
Original name: Nutrient broth

Overview

External ontology
TOGO:M2783 — Nutrient broth
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2783

Composition

flowchart LR
    CultureMech_009332["nutrient_broth"]
    ing0_1M_MgSO4["1M MgSO4"]
    CultureMech_009332 --> ing0_1M_MgSO4
    ing1_20__Glucose["20% Glucose"]
    CultureMech_009332 --> ing1_20__Glucose
    ing2_Distilled_water["Distilled water"]
    CultureMech_009332 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_009332 --> ing3_Yeast_extract
    ing4_KH2PO4["KH2PO4"]
    CultureMech_009332 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_K2HPO4__anhydrous_["K2HPO4 (anhydrous)"]
    CultureMech_009332 --> ing5_K2HPO4__anhydrous_
    ing6_Nutrient_broth["Nutrient broth"]
    CultureMech_009332 --> ing6_Nutrient_broth

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 1M MgSO4 1 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component
2 20% Glucose 25 G_PER_L Role: Carbon source; Properties: Defined component, Organic compound, Solution
3 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
4 Yeast extract 2 G_PER_L Role: Nutrient source; Properties: Undefined component
5 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
6 K2HPO4 (anhydrous) 2 G_PER_L Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
7 Nutrient broth 8 G_PER_L Properties: Undefined component, Complex component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.297108Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2783
2026-01-28T19:48:08.834437Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:05.700726Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009332
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.615745+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nutrient broth" to "nutrient_broth" for consistent matching
2026-06-05T07:40:39.312463+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:49.943742+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:13.912873+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint b38bd866096c80b817afa0fb60bc9678688408710d94f55306ab096b6f789d15