Overview
- External ontology
- TOGO:M2783 — Nutrient broth
- Applications
-
- Microbial cultivation
- Notes
- TOGO M2783 records the nutrient broth used to grow Enterobacter cloacae strain EcWSU1 overnight for Schroeder et al., Plant Disease 94:236-243. The formulation is, per liter of distilled water, 8.0 g nutrient broth, 2.0 g yeast extract, 2.0 g anhydrous K2HPO4, 0.5 g KH2PO4, 25.0 ml 20% glucose, and 1.0 ml 1 M MgSO4.
Composition
flowchart LR
CultureMech_009332["nutrient_broth"]
ing0_Nutrient_broth["Nutrient broth"]
CultureMech_009332 --> ing0_Nutrient_broth
ing1_Yeast_extract["Yeast extract"]
CultureMech_009332 --> ing1_Yeast_extract
ing2_K2HPO4__anhydrous_["K2HPO4 (anhydrous)"]
CultureMech_009332 --> ing2_K2HPO4__anhydrous_
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4__anhydrous_ -.-> CHEBI_131527
ing3_KH2PO4["KH2PO4"]
CultureMech_009332 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Distilled_water["Distilled water"]
CultureMech_009332 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Nutrient broth | 8.0 G_PER_L | TOGO M2783 lists 8.0 g/L Difco nutrient broth powder; the complex product remains intentionally unmapped. | |
| 2 | Yeast extract | 2.0 G_PER_L |
FOODON:03315426
yeast extract
|
TOGO M2783 lists 2.0 g/L yeast extract. |
| 3 |
K2HPO4 (anhydrous)
HO4P.2K 174.174 g/mol
|
2.0 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
TOGO M2783 lists 2.0 g/L anhydrous K2HPO4. |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
TOGO M2783 lists 0.5 g/L KH2PO4. |
| 5 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
TOGO M2783 lists the formulation per liter of distilled water. |
Preparation
- Per liter of distilled water, dissolve 8.0 g Nutrient broth, 2.0 g yeast extract, 2.0 g anhydrous K2HPO4, and 0.5 g KH2PO4, then add 25.0 ml 20% glucose and 1.0 ml 1 M MgSO4.
Solutions
20% Glucose
Dose: 25.0 ML_PER_L
TOGO M2783 lists 25.0 ml/L 20% glucose solution.
1M MgSO4
Dose: 1.0 ML_PER_L
TOGO M2783 lists 1.0 ml/L 1 M MgSO4 solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.297108Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2783 |
| 2026-01-28T19:48:08.834437Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:05.700726Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009332 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.615745+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nutrient broth" to "nutrient_broth" for consistent matching |
| 2026-06-05T07:40:39.312463+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.943742+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m2783_score15.py | RESOLVED_TOGO_M2783_SCORE15 | TOGO M2783 records the nutrient broth used to grow Enterobacter cloacae strain EcWSU1 overnight for Schroeder et al., Plant Disease 94:236-243. The formulation is, per liter of distilled water, 8.0 g nutrient broth, 2.0 g yeast extract, 2.0 g anhydrous K2HPO4, 0.5 g KH2PO4, 25.0 ml 20% glucose, and 1.0 ml 1 M MgSO4. Corrected the imported 1 M MgSO4, 20% glucose, and distilled-water units; moved glucose and MgSO4 stocks to solutions; grounded the machine-usable salts, glucose, water, and yeast extract; and left the Difco nutrient broth product intentionally unmapped. |