methanosaeta_thermophila_medium

CultureMech:009347 archaea COMPLEX LIQUID
Original name: Methanosaeta Thermophila Medium

Overview

External ontology
TOGO:M279 — Methanosaeta Thermophila Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M279 Original source: JCM - JCM_M285 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=285

Composition

flowchart LR
    CultureMech_009347["methanosaeta_thermophila_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009347 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NH4Cl["NH4Cl"]
    CultureMech_009347 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_K2HPO4["K2HPO4"]
    CultureMech_009347 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Resazurin["Resazurin"]
    CultureMech_009347 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_009347 --> ing4_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_6H2O -.-> CHEBI_86345
    ing5_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009347 --> ing5_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing5_Carbon_dioxide_gas -.-> CHEBI_16526
    ing6_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009347 --> ing6_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing6_Nitrogen_gas -.-> CHEBI_17997
    ing7_CO2_gas["CO2 gas"]
    CultureMech_009347 --> ing7_CO2_gas

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
3 K2HPO4
HO4P.2K 174.174 g/mol
0.4 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
4 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component
5 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
6 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
7 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
8 CO2 gas variable VARIABLE Properties: Gas

Solutions

Trace minerals (see Medium [M142])

Dose: 10 G_PER_L

Cross-reference to Medium M142

5% NaHCO3 solution

Dose: 20 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

1% CaCl2・2H2O solution

Dose: 10 G_PER_L

Role: Mineral source; Properties: Defined component, Inorganic compound, Solution

33% Sodium acetate solution

Dose: 10 G_PER_L

Role: Buffer; Properties: Defined component, Organic compound, Solution

1.42% Coenzyme M solution

Dose: 10 G_PER_L

Role: Growth factor; Properties: Defined component, Organic compound, Solution

5% Na2S・9H2O solution

Dose: 5 G_PER_L

Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.752538Z togo-import Imported from TOGO Medium Source: JCM, ID: M279
2026-01-28T19:48:20.855269Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: CO2 gas
2026-03-09T08:04:34.607810+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 7 solution(s) from ingredients
2026-03-13T06:20:05.785815Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009347
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.704343+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Methanosaeta Thermophila Medium" to "methanosaeta_thermophila_medium" for consistent matching
2026-05-16T09:03:16.077123+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:39.342411+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:49.954385+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:27.277699+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint c680f26f491e29c826c479c8be2110cb4a655f9c50b23e3ffa8a608d2f404e14