Overview
- External ontology
- TOGO:M279 — Methanosaeta Thermophila Medium
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- JCM Medium 285 prepares Methanosaeta Thermophila Medium with NH4Cl, K2HPO4, MgCl2, resazurin, Trace minerals from JCM 151, distilled water, and sterile post-autoclave NaHCO3, CaCl2, acetate, vitamin, Coenzyme M, and Na2S stocks.
Composition
flowchart LR
CultureMech_009347["methanosaeta_thermophila_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_009347 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_K2HPO4["K2HPO4"]
CultureMech_009347 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgCl2_x_6H2O["MgCl2 x 6H2O"]
CultureMech_009347 --> ing2_MgCl2_x_6H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6H2O -.-> CHEBI_86345
ing3_Resazurin["Resazurin"]
CultureMech_009347 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_Distilled_water["Distilled water"]
CultureMech_009347 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
ing5_N2["N2"]
CultureMech_009347 --> ing5_N2
CHEBI_17997["CHEBI:17997"]
ing5_N2 -.-> CHEBI_17997
ing6_CO2["CO2"]
CultureMech_009347 --> ing6_CO2
CHEBI_16526["CHEBI:16526"]
ing6_CO2 -.-> CHEBI_16526
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
JCM Medium 285 lists 0.5 G_PER_L NH4Cl. |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
JCM Medium 285 lists 0.4 G_PER_L K2HPO4. |
| 3 |
MgCl2 x 6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
JCM Medium 285 lists 0.1 G_PER_L MgCl2 x 6H2O. |
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
1.0 MG_PER_L |
CHEBI:8806
Resazurin
|
JCM Medium 285 lists 1.0 MG_PER_L Resazurin. |
| 5 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
JCM Medium 285 lists 1.0 L Distilled water. |
| 6 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
JCM Medium 285 cools the boiled medium under N2-CO2 (80:20, v/v). |
| 7 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
JCM Medium 285 cools the boiled medium under N2-CO2 and adds CO2 to 30% of the gas phase. |
Preparation
- Mix direct ingredients and Trace minerals, boil for 5 minutes, and cool under N2-CO2.
- Dispense under N2-CO2, seal with butyl rubber stoppers, and autoclave.
- After autoclaving, add sterile anaerobic NaHCO3, CaCl2, acetate, vitamin, Coenzyme M, and Na2S stocks.
- Add filter-sterilized CO2 to 30% of the gas phase and check final pH at 6.5.
Solutions
Trace minerals
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nitrilotriacetic acid | 1.5 G_PER_L | JCM Medium 151 prints this component in its stock solution. |
| MgSO4 x 7H2O | 3.0 G_PER_L | JCM Medium 151 prints this component in its stock solution. |
| MnSO4 x H2O | 0.5 G_PER_L | JCM Medium 151 prints this component in its stock solution. |
| NaCl | 1.0 G_PER_L | JCM Medium 151 prints this component in its stock solution. |
| FeSO4 x 7H2O | 0.1 G_PER_L | JCM Medium 151 prints this component in its stock solution. |
| CoSO4 x 7H2O | 0.1 G_PER_L | JCM Medium 151 prints this component in its stock solution. |
| CaCl2 x 2H2O | 0.1 G_PER_L | JCM Medium 151 prints this component in its stock solution. |
| ZnSO4 x 7H2O | 0.1 G_PER_L | JCM Medium 151 prints this component in its stock solution. |
| CuSO4 x 5H2O | 0.01 G_PER_L | JCM Medium 151 prints this component in its stock solution. |
| AlK(SO4)2 | 0.01 G_PER_L | JCM Medium 151 prints this component in its stock solution. |
| H3BO3 | 0.01 G_PER_L | JCM Medium 151 prints this component in its stock solution. |
| Na2MoO4 x 2H2O | 0.01 G_PER_L | JCM Medium 151 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 151 prints this component in its stock solution. |
JCM Medium 285 adds 10.0 ml/L Trace minerals from JCM Medium 151.
5% NaHCO3 solution
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaHCO3 | 5 PERCENT_W_V | JCM Medium 285 identifies this as a 5% w/v stock solution. |
JCM Medium 285 adds 20.0 ml/L sterile anaerobic 5% NaHCO3 solution.
1% CaCl2 x 2H2O solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CaCl2 x 2H2O | 1 PERCENT_W_V | JCM Medium 285 identifies this as a 1% w/v stock solution. |
JCM Medium 285 adds 10.0 ml/L sterile anaerobic 1% CaCl2 x 2H2O solution.
33% Sodium acetate solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium acetate | 33 PERCENT_W_V | JCM Medium 285 identifies this as a 33% w/v stock solution. |
JCM Medium 285 adds 10.0 ml/L sterile anaerobic 33% Sodium acetate solution.
Trace vitamins
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Folic acid | 2.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Pyridoxine HCl | 10.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Thiamine HCl | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Riboflavin | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Nicotinic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Calcium pantothenate | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Vitamin B12 | 0.1 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Lipoic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 197 prints this component in its stock solution. |
JCM Medium 285 adds 10.0 ml/L Trace vitamins from JCM Medium 197.
1.42% Coenzyme M solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Coenzyme M | 1.42 PERCENT_W_V | JCM Medium 285 identifies this as a 1.42% w/v stock solution. |
JCM Medium 285 adds 10.0 ml/L sterile anaerobic 1.42% Coenzyme M solution.
5% Na2S x 9H2O solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9H2O | 5 PERCENT_W_V | JCM Medium 285 identifies this as a 5% w/v stock solution. |
JCM Medium 285 adds 5.0 ml/L sterile anaerobic 5% Na2S x 9H2O solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.752538Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M279 |
| 2026-01-28T19:48:20.855269Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: CO2 gas |
| 2026-03-09T08:04:34.607810+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T06:20:05.785815Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009347 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.704343+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanosaeta Thermophila Medium" to "methanosaeta_thermophila_medium" for consistent matching |
| 2026-05-16T09:03:16.077123+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:39.342411+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.954385+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-10T00:00:00-07:00 | repair_togo_jcm_archaea_score15.py | RESOLVED_TOGO_JCM_ARCHAEA_SCORE15 | JCM Medium 285 prepares Methanosaeta Thermophila Medium with NH4Cl, K2HPO4, MgCl2, resazurin, Trace minerals from JCM 151, distilled water, and sterile post-autoclave NaHCO3, CaCl2, acetate, vitamin, Coenzyme M, and Na2S stocks. |