Overview
- External ontology
- TOGO:M2804 — MRS broth with 0.05% (w/v) cysteine hydrochloride
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2804
Composition
flowchart LR
CultureMech_009352["mrs_broth_with_0_05_w_v_cysteine_hydrochloride"]
ing0_Cysteine_hydrochloride["Cysteine hydrochloride"]
CultureMech_009352 --> ing0_Cysteine_hydrochloride
CHEBI_91248["CHEBI:91248"]
ing0_Cysteine_hydrochloride -.-> CHEBI_91248
ing1_MRS_broth__Oxoid_["MRS broth (Oxoid)"]
CultureMech_009352 --> ing1_MRS_broth__Oxoid_
ing2_Distilled_water["Distilled water"]
CultureMech_009352 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_Magnesium_sulphate_7H2O["Magnesium sulphate 7H2O"]
CultureMech_009352 --> ing3_Magnesium_sulphate_7H2O
ing4_Yeast_extract["Yeast extract"]
CultureMech_009352 --> ing4_Yeast_extract
ing5_Dipotassium_hydrogen_phosphate["Dipotassium hydrogen phosphate"]
CultureMech_009352 --> ing5_Dipotassium_hydrogen_phosphate
CHEBI_131527["CHEBI:131527"]
ing5_Dipotassium_hydrogen_phosphate -.-> CHEBI_131527
ing6_Manganese_sulphate_4H2O["Manganese sulphate 4H2O"]
CultureMech_009352 --> ing6_Manganese_sulphate_4H2O
ing7_Sorbitan_mono_oleate["Sorbitan mono-oleate"]
CultureMech_009352 --> ing7_Sorbitan_mono_oleate
ing8_Triammonium_citrate["Triammonium citrate"]
CultureMech_009352 --> ing8_Triammonium_citrate
CHEBI_63037["CHEBI:63037"]
ing8_Triammonium_citrate -.-> CHEBI_63037
ing9_Sodium_acetate_3H2O["Sodium acetate 3H2O"]
CultureMech_009352 --> ing9_Sodium_acetate_3H2O
ing10_Glucose["Glucose"]
CultureMech_009352 --> ing10_Glucose
CHEBI_17234["CHEBI:17234"]
ing10_Glucose -.-> CHEBI_17234
ing11__Lab_Lemco__powder["`Lab-Lemco’ powder"]
CultureMech_009352 --> ing11__Lab_Lemco__powder
ing12_Peptone["Peptone"]
CultureMech_009352 --> ing12_Peptone
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Cysteine hydrochloride
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.05 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component |
| 2 | MRS broth (Oxoid) | 1 G_PER_L | ||
| 3 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 4 | Magnesium sulphate 7H2O | 0.2 G_PER_L | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound | |
| 5 | Yeast extract | 4 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
Dipotassium hydrogen phosphate
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 7 | Manganese sulphate 4H2O | 0.05 G_PER_L | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound | |
| 8 | Sorbitan mono-oleate | 1 G_PER_L | Role: Carbon source; Properties: Simple component, Organic compound, Defined component | |
| 9 |
Triammonium citrate
C6H5O7.3H4N 243.216 g/mol
|
2 G_PER_L |
CHEBI:63037
triammonium citrate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 10 | Sodium acetate 3H2O | 5 G_PER_L | Role: Buffer; Properties: Simple component, Organic compound, Defined component | |
| 11 |
Glucose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 12 | `Lab-Lemco’ powder | 8 G_PER_L | Properties: Undefined component | |
| 13 | Peptone | 10 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mrs_broth_with_0_05_w_v_cysteine_hydrochloride (CultureMech:009352)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.316150Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2804 |
| 2026-01-28T19:48:14.647117Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:05.815692Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009352 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.736861+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MRS broth with 0.05% (w/v) cysteine hydrochloride" to "mrs_broth_with_0_05_w_v_cysteine_hydrochloride" for consistent matching |
| 2026-06-05T07:41:38.454753+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:50.461508+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fb90634a704abf7575055502d31b6d301df379bda1f8dd1f56b8fda3c748d302 |