mrs_broth_with_0_05_w_v_cysteine_hydrochloride

CultureMech:009352 bacterial COMPLEX LIQUID
Original name: MRS broth with 0.05% (w/v) cysteine hydrochloride

Overview

External ontology
TOGO:M2804 — MRS broth with 0.05% (w/v) cysteine hydrochloride
Applications
  • Microbial cultivation
pH range
{'min': 6.0, 'max': 6.4}
Notes
TOGO M2804 lists the TOGO M2242 MRS broth (Oxoid) formula supplemented with 0.05% w/v cysteine hydrochloride at pH 6.2 +/- 0.2.

Composition

flowchart LR
    CultureMech_009352["mrs_broth_with_0_05_w_v_cysteine_hydrochloride"]
    ing0_Cysteine_hydrochloride["Cysteine hydrochloride"]
    CultureMech_009352 --> ing0_Cysteine_hydrochloride
    CHEBI_91248["CHEBI:91248"]
    ing0_Cysteine_hydrochloride -.-> CHEBI_91248
    ing1_Distilled_water["Distilled water"]
    CultureMech_009352 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_Magnesium_sulphate_7H2O["Magnesium sulphate 7H2O"]
    CultureMech_009352 --> ing2_Magnesium_sulphate_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_Magnesium_sulphate_7H2O -.-> CHEBI_31795
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_009352 --> ing3_Yeast_extract
    ing4_Dipotassium_hydrogen_phosphate["Dipotassium hydrogen phosphate"]
    CultureMech_009352 --> ing4_Dipotassium_hydrogen_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing4_Dipotassium_hydrogen_phosphate -.-> CHEBI_131527
    ing5_Manganese_sulphate_4H2O["Manganese sulphate 4H2O"]
    CultureMech_009352 --> ing5_Manganese_sulphate_4H2O
    CHEBI_86358["CHEBI:86358"]
    ing5_Manganese_sulphate_4H2O -.-> CHEBI_86358
    ing6_Sorbitan_mono_oleate["Sorbitan mono-oleate"]
    CultureMech_009352 --> ing6_Sorbitan_mono_oleate
    ing7_Triammonium_citrate["Triammonium citrate"]
    CultureMech_009352 --> ing7_Triammonium_citrate
    CHEBI_63037["CHEBI:63037"]
    ing7_Triammonium_citrate -.-> CHEBI_63037
    ing8_Sodium_acetate_3H2O["Sodium acetate 3H2O"]
    CultureMech_009352 --> ing8_Sodium_acetate_3H2O
    CHEBI_32138["CHEBI:32138"]
    ing8_Sodium_acetate_3H2O -.-> CHEBI_32138
    ing9_Glucose["Glucose"]
    CultureMech_009352 --> ing9_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing9_Glucose -.-> CHEBI_17234
    ing10_Lab_Lemco_powder__Oxoid_["Lab-Lemco powder (Oxoid)"]
    CultureMech_009352 --> ing10_Lab_Lemco_powder__Oxoid_
    ing11_Peptone["Peptone"]
    CultureMech_009352 --> ing11_Peptone

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Cysteine hydrochloride
C3H7NO2S.H2O.HCl 175.637 g/mol
0.05 PERCENT_W_V CHEBI:91248
L-cysteine hydrochloride hydrate
 TOGO M2804 lists 0.05 % w/v Cysteine hydrochloride.
2 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M2804 lists 1.0 L Distilled water.
3 Magnesium sulphate 7H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 TOGO M2804 lists 0.2 g/L Magnesium sulphate 7H2O.
4 Yeast extract 4.0 G_PER_L FOODON:03315426
yeast extract
 TOGO M2804 lists 4.0 g/L Yeast extract.
5 Dipotassium hydrogen phosphate
HO4P.2K 174.174 g/mol
2.0 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 TOGO M2804 lists 2.0 g/L Dipotassium hydrogen phosphate.
6 Manganese sulphate 4H2O
4H2O.Mn.O4S 223.061 g/mol
0.05 G_PER_L CHEBI:86358
manganese(II) sulfate tetrahydrate
 TOGO M2804 lists 0.05 g/L Manganese sulphate 4H2O.
7 Sorbitan mono-oleate 1.0 ML_PER_L TOGO M2804 lists 1.0 ml/L Sorbitan mono-oleate.
8 Triammonium citrate
C6H5O7.3H4N 243.216 g/mol
2.0 G_PER_L CHEBI:63037
triammonium citrate
 TOGO M2804 lists 2.0 g/L Triammonium citrate.
9 Sodium acetate 3H2O
C2H3O2.3H2O.Na 136.079 g/mol
5.0 G_PER_L CHEBI:32138
sodium acetate trihydrate
 TOGO M2804 lists 5.0 g/L Sodium acetate 3H2O.
10 Glucose
C6H12O6 180.156 g/mol
20.0 G_PER_L CHEBI:17234
glucose
 TOGO M2804 lists 20.0 g/L Glucose.
11 Lab-Lemco powder (Oxoid) 8.0 G_PER_L TOGO M2804 lists 8.0 g/L Lab-Lemco powder (Oxoid).
12 Peptone 10.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
 TOGO M2804 lists 10.0 g/L Peptone.

Preparation

  1. Prepare the MRS broth (Oxoid) components, add 0.05% w/v cysteine hydrochloride, and adjust pH to 6.2 +/- 0.2 at 25 C.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.316150Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2804
2026-01-28T19:48:14.647117Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:05.815692Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009352
2026-03-17T06:29:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.736861+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MRS broth with 0.05% (w/v) cysteine hydrochloride" to "mrs_broth_with_0_05_w_v_cysteine_hydrochloride" for consistent matching
2026-06-05T07:41:38.454753+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2242_m2796_m2804_mrs_broths_score15.py RESOLVED_TOGO_M2804_MRS_CYSTEINE_SCORE15 TOGO M2804 lists the TOGO M2242 MRS broth (Oxoid) formula supplemented with 0.05% w/v cysteine hydrochloride at pH 6.2 +/- 0.2. Corrected imported one-liter water units, normalized source gram and milliliter quantities, added the source pH range, and grounded unambiguous components.