Overview
- External ontology
- TOGO:M2811 — Brain heart infusion (BHI) broth containing C. difficile supplement and 0.04% cysteine
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2811 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009359["brain_heart_infusion_bhi_broth_containing_c_difficile_supplement_an..."]
ing0_Cysteine["Cysteine"]
CultureMech_009359 --> ing0_Cysteine
CHEBI_15356["CHEBI:15356"]
ing0_Cysteine -.-> CHEBI_15356
ing1_Calf_brains["Calf brains"]
CultureMech_009359 --> ing1_Calf_brains
ing2_Beef_heart["Beef heart"]
CultureMech_009359 --> ing2_Beef_heart
ing3_Proteose_peptone["Proteose peptone"]
CultureMech_009359 --> ing3_Proteose_peptone
ing4_Dextrose["Dextrose"]
CultureMech_009359 --> ing4_Dextrose
CHEBI_17634["CHEBI:17634"]
ing4_Dextrose -.-> CHEBI_17634
ing5_Sodium_chloride["Sodium chloride"]
CultureMech_009359 --> ing5_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_chloride -.-> CHEBI_26710
ing6_Disodium_phosphate["Disodium phosphate"]
CultureMech_009359 --> ing6_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing6_Disodium_phosphate -.-> CHEBI_34683
ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009359 --> ing7_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing7_Carbon_dioxide_gas -.-> CHEBI_16526
ing8_Nitrogen_gas["Nitrogen gas"]
CultureMech_009359 --> ing8_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing8_Nitrogen_gas -.-> CHEBI_17997
ing9_Hydrogen_gas["Hydrogen gas"]
CultureMech_009359 --> ing9_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing9_Hydrogen_gas -.-> CHEBI_18276
ing10_C__difficile_supplement["C. difficile supplement"]
CultureMech_009359 --> ing10_C__difficile_supplement
ing11_D_cycloserine["D-cycloserine"]
CultureMech_009359 --> ing11_D_cycloserine
ing12_Cefoxitin["Cefoxitin"]
CultureMech_009359 --> ing12_Cefoxitin
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Cysteine
C3H7NO2S 121.161 g/mol
|
0.04 PERCENT_W_V |
CHEBI:15356
cysteine
|
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component |
| 2 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 3 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 4 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 5 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 6 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 7 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 8 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 9 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 10 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 11 | C. difficile supplement | variable VARIABLE | ||
| 12 | D-cycloserine | variable VARIABLE | Role: Antimicrobial agent | |
| 13 | Cefoxitin | variable VARIABLE | Role: Antimicrobial agent |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- brain_heart_infusion_bhi_broth_containing_c_difficile_supplement_and_0_04_cysteine (CultureMech:009359)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.325690Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2811 |
| 2026-01-28T19:48:38.802614Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: C. difficile supplement; Added default concentration for ingredient: D-cycloserine; Added default concentration for ingredient: Cefoxitin |
| 2026-03-13T06:20:05.863937Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009359 |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.789623+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Brain heart infusion (BHI) broth containing C. difficile supplement and 0.04% cysteine" to "brain_heart_infusion_bhi_broth_containing_c_difficile_supplement_and_0_04_cysteine" for consistent matching |
| 2026-05-16T09:03:26.123368+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:51.439746+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:25.856654+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e36b75f207ee95ac6143abaaf9b6a57a740f0dff56a740dad434b7ab76f1abca |