brain_heart_infusion_bhi_broth_containing_c_difficile_supplement_and_0_04_cysteine

CultureMech:009359 bacterial COMPLEX LIQUID
Original name: Brain heart infusion (BHI) broth containing C. difficile supplement and 0.04% cysteine

Overview

External ontology
TOGO:M2811 — Brain heart infusion (BHI) broth containing C. difficile supplement and 0.04% cysteine
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2811 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_009359["brain_heart_infusion_bhi_broth_containing_c_difficile_supplement_an..."]
    ing0_Cysteine["Cysteine"]
    CultureMech_009359 --> ing0_Cysteine
    CHEBI_15356["CHEBI:15356"]
    ing0_Cysteine -.-> CHEBI_15356
    ing1_Calf_brains["Calf brains"]
    CultureMech_009359 --> ing1_Calf_brains
    ing2_Beef_heart["Beef heart"]
    CultureMech_009359 --> ing2_Beef_heart
    ing3_Proteose_peptone["Proteose peptone"]
    CultureMech_009359 --> ing3_Proteose_peptone
    ing4_Dextrose["Dextrose"]
    CultureMech_009359 --> ing4_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing4_Dextrose -.-> CHEBI_17634
    ing5_Sodium_chloride["Sodium chloride"]
    CultureMech_009359 --> ing5_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing5_Sodium_chloride -.-> CHEBI_26710
    ing6_Disodium_phosphate["Disodium phosphate"]
    CultureMech_009359 --> ing6_Disodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing6_Disodium_phosphate -.-> CHEBI_34683
    ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009359 --> ing7_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing7_Carbon_dioxide_gas -.-> CHEBI_16526
    ing8_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009359 --> ing8_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing8_Nitrogen_gas -.-> CHEBI_17997
    ing9_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009359 --> ing9_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing9_Hydrogen_gas -.-> CHEBI_18276
    ing10_C__difficile_supplement["C. difficile supplement"]
    CultureMech_009359 --> ing10_C__difficile_supplement
    ing11_D_cycloserine["D-cycloserine"]
    CultureMech_009359 --> ing11_D_cycloserine
    CHEBI_40009["CHEBI:40009"]
    ing11_D_cycloserine -.-> CHEBI_40009
    ing12_Cefoxitin["Cefoxitin"]
    CultureMech_009359 --> ing12_Cefoxitin
    CHEBI_209807["CHEBI:209807"]
    ing12_Cefoxitin -.-> CHEBI_209807

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Cysteine
C3H7NO2S 121.161 g/mol
0.04 PERCENT_W_V CHEBI:15356
cysteine
 Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component
2 Calf brains
Difco/BD Brain Heart Infusion 237500
variable VARIABLE UBERON:0000955
brain
 infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product
3 Beef heart
Difco/BD Brain Heart Infusion 237500
variable VARIABLE UBERON:0000948
heart
 infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product
4 Proteose peptone
Difco/BD Brain Heart Infusion 237500
10.0 G_PER_L MICRO:0000180 (source link unavailable)
proteose peptone
 Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product
5 Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
2.0 G_PER_L CHEBI:17634
D-glucose
 Carbon/energy source; Constituent of Brain Heart Infusion commercial product
6 Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product
7 Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
2.5 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product
8 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
9 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
10 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス
11 C. difficile supplement variable VARIABLE
12 D-cycloserine
C3H6N2O2 102.093 g/mol
variable VARIABLE CHEBI:40009
D-cycloserine
 Role: Antimicrobial agent
13 Cefoxitin
C16H17N3O7S2 427.46 g/mol
variable VARIABLE CHEBI:209807
cefoxitin
 Role: Antimicrobial agent

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.325690Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2811
2026-01-28T19:48:38.802614Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: C. difficile supplement; Added default concentration for ingredient: D-cycloserine; Added default concentration for ingredient: Cefoxitin
2026-03-13T06:20:05.863937Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009359
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.789623+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Brain heart infusion (BHI) broth containing C. difficile supplement and 0.04% cysteine" to "brain_heart_infusion_bhi_broth_containing_c_difficile_supplement_and_0_04_cysteine" for consistent matching
2026-05-16T09:03:26.123368+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:51.439746+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:01:35.947214+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 2 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).