Overview
- External ontology
- TOGO:M2880 — Middlebrook 7H9 (Difco) liquid medium supplemented with Middlebrook albumin/dextrose/catalase and Tween 80
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2880 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009415["middlebrook_7h9_difco_liquid_medium_supplemented_with_middlebrook_a..."]
ing0_Distilled_water["Distilled water"]
CultureMech_009415 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Tween_80__BD_["Tween 80 (BD)"]
CultureMech_009415 --> ing1_Tween_80__BD_
ing2_Middlebrook_7H9_broth__Difco_["Middlebrook 7H9 broth (Difco)"]
CultureMech_009415 --> ing2_Middlebrook_7H9_broth__Difco_
ing3_Tryptone["Tryptone"]
CultureMech_009415 --> ing3_Tryptone
ing4_Yeast_extract["Yeast extract"]
CultureMech_009415 --> ing4_Yeast_extract
ing5_Sodium_chloride["Sodium chloride"]
CultureMech_009415 --> ing5_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_chloride -.-> CHEBI_26710
ing6_Dextrose["Dextrose"]
CultureMech_009415 --> ing6_Dextrose
CHEBI_17634["CHEBI:17634"]
ing6_Dextrose -.-> CHEBI_17634
ing7_Catalase["Catalase"]
CultureMech_009415 --> ing7_Catalase
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
500.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 450.0, 50.0] |
| 2 | Tween 80 (BD) | 0.05 PERCENT_W_V | Role: Surfactant | |
| 3 | Middlebrook 7H9 broth (Difco) | 2.35 G_PER_L | Properties: Complex component | |
| 4 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 5 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 6 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 7 |
Dextrose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 8 | Catalase | 0.0015 G_PER_L | Properties: Simple component, Defined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- middlebrook_7h9_difco_liquid_medium_supplemented_with_middlebrook_albumin_dextrose_catalase_and_tween_80 (CultureMech:009415)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:57.374710Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2880 |
| 2026-01-28T19:48:32.665071Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:25.757020Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:06.102022Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009415 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.051398+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Middlebrook 7H9 (Difco) liquid medium supplemented with Middlebrook albumin/dextrose/catalase and Tween 80" to "middlebrook_7h9_difco_liquid_medium_supplemented_with_middlebrook_albumin_dextrose_catalase_and_tween_80" for consistent matching |
| 2026-06-05T07:41:34.001673+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:45.542345+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 11954cc76bcd72fd6447863f19dc59e014d2b51465e1f64ee710aa48061311d9 |
| 2026-08-15T20:09:30.449936+00:00 | dedupe_identical_ingredients.py | REMOVED_DUPLICATE_INGREDIENT_ROWS | Removed 3 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Sodium chloride, Tryptone, Yeast extract. |