middlebrook_7h9_difco_liquid_medium_supplemented_with_middlebrook_albumin_dextrose_catalase_and_tween_80

CultureMech:009415 bacterial COMPLEX LIQUID
Original name: Middlebrook 7H9 (Difco) liquid medium supplemented with Middlebrook albumin/dextrose/catalase and Tween 80

Overview

External ontology
TOGO:M2880 — Middlebrook 7H9 (Difco) liquid medium supplemented with Middlebrook albumin/dextrose/catalase and Tween 80
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2880 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. False LB constituent expansion is withdrawn. Source-specific amounts and preparation boundaries supersede flattened concentrations. Existing chemical annotations and earlier history are preserved, not recertified; no organism-growth association is added. The quoted cultivation sentence assigns ADC/Tween to liquid medium, but the original publication is unidentified and uninspected. No organism-growth association is curated from this secondary quotation.

Composition

flowchart LR
    CultureMech_009415["middlebrook_7h9_difco_liquid_medium_supplemented_with_middlebrook_a..."]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009415 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Tween_80__BD_["Tween 80 (BD)"]
    CultureMech_009415 --> ing1_Tween_80__BD_
    ing2_Middlebrook_7H9_broth__Difco_["Middlebrook 7H9 broth (Difco)"]
    CultureMech_009415 --> ing2_Middlebrook_7H9_broth__Difco_

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
0.45 L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 450.0, 50.0] Source amount: 450 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 450 ml = 0.45 L. Earlier merged-quantity text is retained only as historical provenance; the separated source stages supersede it.
2 Tween 80 (BD) Role: Surfactant Source amount: 0.05 %; scope: main preparation input. Percentage basis is unspecified; the earlier w/v or g/L assertion is withdrawn, not replaced with an assumed basis.
3 Middlebrook 7H9 broth (Difco) Properties: Complex component Source amount: 2.35 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit.

Solutions

Middlebrook albumin/dextrose/catalase (Difco)

Dose: 0.05 L

Stock composition
IngredientConcentrationNotes
Distilled water 0.05 L Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 450.0, 50.0] Source amount: 50 ml; scope: Middlebrook albumin/dextrose/catalase (Difco) stock preparation. Volume-only input/dose, not a final-volume marker; 50 ml = 0.05 L. Earlier merged-quantity text is retained only as historical provenance; the separated source stages supersede it.
Dextrose Role: Carbon source; Properties: Defined component, Organic compound, Simple component Source amount: 1 g; scope: Middlebrook albumin/dextrose/catalase (Difco) stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit.
Bovine albumin fraction V Source amount: 2.5 g; scope: Middlebrook albumin/dextrose/catalase (Difco) stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit.
Catalase Properties: Simple component, Defined component Source amount: 0.0015 g; scope: Middlebrook albumin/dextrose/catalase (Difco) stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit.
Source amount: 50 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 50 ml = 0.05 L. Inline enrichment composition is separate from its dose and from finished-medium concentrations.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable).
2026-01-27T06:35:57.374710Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2880
2026-01-28T19:48:32.665071Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:25.757020Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:06.102022Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009415
2026-03-17T06:30:34.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.051398+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Middlebrook 7H9 (Difco) liquid medium supplemented with Middlebrook albumin/dextrose/catalase and Tween 80" to "middlebrook_7h9_difco_liquid_medium_supplemented_with_middlebrook_albumin_dextrose_catalase_and_tween_80" for consistent matching
2026-06-05T07:41:34.001673+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-15T20:08:38.760585+00:00 dedupe_identical_ingredients.py REMOVED_DUPLICATE_INGREDIENT_ROWS Removed 3 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Sodium chloride, Tryptone, Yeast extract.
2026-10-09T00:43:54.768901+00:00 codex RESTORED_MIDDLEBROOK_INGREDIENT_AND_STOCK_SCOPES_WITH_EVIDENCE Restore albumin and separate basal inputs, commercial-base detail and ADC/OADC stock amounts/doses. Correct volume/mass confusion; retain unsupported mass/percent bases and source-version conflicts explicitly. Preserve IDs and all prior history.