Overview
- External ontology
- TOGO:M2888 — Modified Hayflick broth medium containing 20% fetal bovine serum
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2888
Composition
flowchart LR
CultureMech_009423["modified_hayflick_broth_medium_containing_20_fetal_bovine_serum"]
ing0_0_5__phenol_red__Flow_["0.5% phenol red (Flow)"]
CultureMech_009423 --> ing0_0_5__phenol_red__Flow_
ing1_Distilled_water["Distilled water"]
CultureMech_009423 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_D_glucose["D-glucose"]
CultureMech_009423 --> ing2_D_glucose
CHEBI_17634["CHEBI:17634"]
ing2_D_glucose -.-> CHEBI_17634
ing3_Liquid_yeast_extract__Flow_["Liquid yeast extract (Flow)"]
CultureMech_009423 --> ing3_Liquid_yeast_extract__Flow_
ing4_Fetal_bovine_serum__Flow___heat_inactivated["Fetal bovine serum (Flow), heat-inactivated"]
CultureMech_009423 --> ing4_Fetal_bovine_serum__Flow___heat_inactivated
ing5_PPLO_broth_base_without_crystal_violet__Difco_["PPLO broth base without crystal violet (Difco)"]
CultureMech_009423 --> ing5_PPLO_broth_base_without_crystal_violet__Difco_
ing6_Penicillin_G__Parke__Davis___Co_["Penicillin G (Parke, Davis & Co)"]
CultureMech_009423 --> ing6_Penicillin_G__Parke__Davis___Co_
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 0.5% phenol red (Flow) | 4 G_PER_L | Role: pH indicator; Properties: Defined component, Organic compound, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
D-glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 4 | Liquid yeast extract (Flow) | 100 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 | Fetal bovine serum (Flow), heat-inactivated | 200 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 | PPLO broth base without crystal violet (Difco) | 21 G_PER_L | Properties: Undefined component, Complex component | |
| 7 | Penicillin G (Parke, Davis & Co) | variable VARIABLE | Role: Antimicrobial agent; Properties: Defined component, Simple component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_hayflick_broth_medium_containing_20_fetal_bovine_serum (CultureMech:009423)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.382576Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2888 |
| 2026-01-28T19:48:26.010883Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Penicillin G (Parke, Davis & Co) |
| 2026-03-13T06:20:06.132245Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009423 |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.084071+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Hayflick broth medium containing 20% fetal bovine serum" to "modified_hayflick_broth_medium_containing_20_fetal_bovine_serum" for consistent matching |
| 2026-05-16T09:04:25.410858+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:36.690982+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:48.362081+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 93a1a85c9ec508072c651a93fb573c6df67553cb165f5e44339db50a314eabbf |