modified_hayflick_broth_medium_containing_20_fetal_bovine_serum

CultureMech:009423 bacterial COMPLEX LIQUID
Original name: Modified Hayflick broth medium containing 20% fetal bovine serum

Overview

External ontology
TOGO:M2888 — Modified Hayflick broth medium containing 20% fetal bovine serum
Applications
  • Microbial cultivation
pH
7.8
Notes
TOGO M2888 records Modified Hayflick broth medium containing 20% fetal bovine serum as 4 ml/L 0.5% phenol red, 1 L distilled water, 5 g/L D-glucose, 100 ml/L Liquid yeast extract (Flow), 200 ml/L heat-inactivated fetal bovine serum from Flow, 21 g/L PPLO broth base without crystal violet from Difco, 5 x 10^5 U/L Penicillin G, pH 7.8, and growth at 37 C.

Composition

flowchart LR
    CultureMech_009423["modified_hayflick_broth_medium_containing_20_fetal_bovine_serum"]
    ing0_0_5__phenol_red__Flow_["0.5% phenol red (Flow)"]
    CultureMech_009423 --> ing0_0_5__phenol_red__Flow_
    ing1_Distilled_water["Distilled water"]
    CultureMech_009423 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_D_glucose["D-glucose"]
    CultureMech_009423 --> ing2_D_glucose
    CHEBI_17634["CHEBI:17634"]
    ing2_D_glucose -.-> CHEBI_17634
    ing3_Liquid_yeast_extract__Flow_["Liquid yeast extract (Flow)"]
    CultureMech_009423 --> ing3_Liquid_yeast_extract__Flow_
    ing4_Fetal_bovine_serum__Flow___heat_inactivated["Fetal bovine serum (Flow), heat-inactivated"]
    CultureMech_009423 --> ing4_Fetal_bovine_serum__Flow___heat_inactivated
    ing5_PPLO_broth_base_without_crystal_violet__Difco_["PPLO broth base without crystal violet (Difco)"]
    CultureMech_009423 --> ing5_PPLO_broth_base_without_crystal_violet__Difco_
    ing6_Penicillin_G__Parke__Davis___Co_["Penicillin G (Parke, Davis & Co)"]
    CultureMech_009423 --> ing6_Penicillin_G__Parke__Davis___Co_
    CHEBI_51765["CHEBI:51765"]
    ing6_Penicillin_G__Parke__Davis___Co_ -.-> CHEBI_51765

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 0.5% phenol red (Flow) 4.0 ML_PER_L TOGO M2888 lists 4 ml/L of 0.5% Phenol red solution from Flow.
2 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M2888 lists 1 L Distilled water.
3 D-glucose
C6H12O6 180.156 g/mol
5.0 G_PER_L CHEBI:17634
D-glucose
 TOGO M2888 lists 5 g/L D-glucose.
4 Liquid yeast extract (Flow) 100.0 ML_PER_L FOODON:03315426
Yeast extract
 TOGO M2888 lists 100 ml/L Liquid yeast extract from Flow.
5 Fetal bovine serum (Flow), heat-inactivated 200.0 ML_PER_L TOGO M2888 lists 200 ml/L heat-inactivated fetal bovine serum from Flow; MIM resolves fetal bovine serum to NCIT:C113696, but NCIT is not an allowed ingredient prefix in the CultureMech schema, so this serum remains intentionally unmapped.
6 PPLO broth base without crystal violet (Difco) 21.0 G_PER_L TOGO M2888 lists 21 g/L PPLO broth base without crystal violet from Difco; this catalog broth is retained as an opaque component.
7 Penicillin G (Parke, Davis & Co)
C16H17N2O4S.Na 356.379 g/mol
variable VARIABLE CHEBI:51765
benzylpenicillin sodium
 TOGO M2888 lists Penicillin G without a schema-compatible mass concentration; the antibiotic identity is grounded but its amount is retained as variable.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.382576Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2888
2026-01-28T19:48:26.010883Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Penicillin G (Parke, Davis & Co)
2026-03-13T06:20:06.132245Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009423
2026-03-17T06:30:20.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.084071+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Hayflick broth medium containing 20% fetal bovine serum" to "modified_hayflick_broth_medium_containing_20_fetal_bovine_serum" for consistent matching
2026-05-16T09:04:25.410858+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:36.690982+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2223_m2888_hayflick_broths_score15.py RESOLVED_TOGO_M2223_M2888_SCORE15 TOGO M2888 records Modified Hayflick broth medium containing 20% fetal bovine serum as 4 ml/L 0.5% phenol red, 1 L distilled water, 5 g/L D-glucose, 100 ml/L Liquid yeast extract (Flow), 200 ml/L heat-inactivated fetal bovine serum from Flow, 21 g/L PPLO broth base without crystal violet from Difco, 5 x 10^5 U/L Penicillin G, pH 7.8, and growth at 37 C. Corrected the imported ml/L and water units, added pH 7.8 and 37 C, grounded the serum, Liquid yeast extract, D-glucose, and Penicillin G, and retained the PPLO broth product and phenol-red stock solution as intentionally unmapped.