Overview
- External ontology
- TOGO:M2950 — MRS (de Man, Rogosa, Sharpe) medium
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'mrs_broth', 'source': 'TOGO', 'source_id': 'TOGO:M2238', 'original_category': 'bacterial'}
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2950
Composition
flowchart LR
CultureMech_009477["mrs_de_man_rogosa_sharpe_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009477 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4.7H2O"]
CultureMech_009477 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_K2HPO4["K2HPO4"]
CultureMech_009477 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_polyoxyethylene_sorbitan_mono_oleate__Tween_80_["polyoxyethylene sorbitan mono-oleate (Tween 80)"]
CultureMech_009477 --> ing3_polyoxyethylene_sorbitan_mono_oleate__Tween_80_
ing4_MnSO4_4H2O["MnSO4.4H2O"]
CultureMech_009477 --> ing4_MnSO4_4H2O
CHEBI_86358["CHEBI:86358"]
ing4_MnSO4_4H2O -.-> CHEBI_86358
ing5_Triammonium_citrate["Triammonium citrate"]
CultureMech_009477 --> ing5_Triammonium_citrate
CHEBI_63037["CHEBI:63037"]
ing5_Triammonium_citrate -.-> CHEBI_63037
ing6_CH3COONa_3H2O["CH3COONa.3H2O"]
CultureMech_009477 --> ing6_CH3COONa_3H2O
ing7_Glucose["Glucose"]
CultureMech_009477 --> ing7_Glucose
CHEBI_17234["CHEBI:17234"]
ing7_Glucose -.-> CHEBI_17234
ing8_Yeast_extract__Difco_or_Oxoid_["Yeast extract (Difco or Oxoid)"]
CultureMech_009477 --> ing8_Yeast_extract__Difco_or_Oxoid_
ing9_Oxoid_peptone["Oxoid peptone"]
CultureMech_009477 --> ing9_Oxoid_peptone
ing10_Lab_Lemco__Oxoid_["Lab-Lemco (Oxoid)"]
CultureMech_009477 --> ing10_Lab_Lemco__Oxoid_
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
|
200 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 4 | polyoxyethylene sorbitan mono-oleate (Tween 80) | 1 G_PER_L | Role: Surfactant; Properties: Organic compound, Simple component, Defined component | |
| 5 |
MnSO4.4H2O
4H2O.Mn.O4S 223.061 g/mol
|
50 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
Triammonium citrate
C6H5O7.3H4N 243.216 g/mol
|
2 G_PER_L |
CHEBI:63037
triammonium citrate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Organic compound |
| 7 | CH3COONa.3H2O | 5 G_PER_L | Role: Buffer; Properties: Defined component, Simple component, Organic compound | |
| 8 |
Glucose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 9 | Yeast extract (Difco or Oxoid) | 5 G_PER_L | Properties: Undefined component | |
| 10 | Oxoid peptone | 10 G_PER_L | Properties: Undefined component | |
| 11 | Lab-Lemco (Oxoid) | 10 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- mrs_broth (CultureMech:008826)
- mrs_de_man_rogosa_sharpe_medium (CultureMech:009477)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.419344Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2950 |
| 2026-01-28T19:48:22.262238Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.332479Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009477 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.309058+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MRS (de Man, Rogosa, Sharpe) medium" to "mrs_de_man_rogosa_sharpe_medium" for consistent matching |
| 2026-06-05T07:41:38.470276+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:27.302995+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:09.188466+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:50.437988+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: mrs_broth, mrs_de_man_rogosa_sharpe_medium |
| 2026-08-06T03:05:50.438024+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint adf818847efb079ca32a1ac67b423b2c562b41787ff5b237e07564b1acba66c8 |