mrs_de_man_rogosa_sharpe_medium

CultureMech:009477 bacterial COMPLEX LIQUID
Original name: MRS (de Man, Rogosa, Sharpe) medium

Overview

External ontology
TOGO:M2950 — MRS (de Man, Rogosa, Sharpe) medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M2950

Composition

flowchart LR
    CultureMech_009477["mrs_de_man_rogosa_sharpe_medium"]
    ing0_distilled_water["distilled water"]
    CultureMech_009477 --> ing0_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_distilled_water -.-> CHEBI_15377
    ing1_MgSO4___7H2O["MgSO4 · 7H2O"]
    CultureMech_009477 --> ing1_MgSO4___7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4___7H2O -.-> CHEBI_31795
    ing2_K2HPO4["K2HPO4"]
    CultureMech_009477 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_polyoxyethylene_sorbitan_mono_oleate__Tween_80_["polyoxyethylene sorbitan mono-oleate (Tween 80)"]
    CultureMech_009477 --> ing3_polyoxyethylene_sorbitan_mono_oleate__Tween_80_
    ing4_MnSO4___4_H2O["MnSO4 · 4 H2O"]
    CultureMech_009477 --> ing4_MnSO4___4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing4_MnSO4___4_H2O -.-> CHEBI_86358
    ing5_triammonium_citrate["triammonium citrate"]
    CultureMech_009477 --> ing5_triammonium_citrate
    CHEBI_63037["CHEBI:63037"]
    ing5_triammonium_citrate -.-> CHEBI_63037
    ing6_CH3COONa_3H2O["CH3COONa.3H2O"]
    CultureMech_009477 --> ing6_CH3COONa_3H2O
    ing7_glucose["glucose"]
    CultureMech_009477 --> ing7_glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_glucose -.-> CHEBI_17234
    ing8_Oxoid_peptone["Oxoid peptone"]
    CultureMech_009477 --> ing8_Oxoid_peptone
    ing9_Lab_Lemco__Oxoid_["Lab-Lemco (Oxoid)"]
    CultureMech_009477 --> ing9_Lab_Lemco__Oxoid_
    ing10_Yeast_extract__Difco_or_Oxoid_["Yeast extract (Difco or Oxoid)"]
    CultureMech_009477 --> ing10_Yeast_extract__Difco_or_Oxoid_

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
200 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
3 K2HPO4
HO4P.2K 174.174 g/mol
2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
4 polyoxyethylene sorbitan mono-oleate (Tween 80) 1 G_PER_L Role: Surfactant; Properties: Organic compound, Simple component, Defined component
5 MnSO4 · 4 H2O
4H2O.Mn.O4S 223.061 g/mol
50 G_PER_L CHEBI:86358
manganese(II) sulfate tetrahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
6 triammonium citrate
C6H5O7.3H4N 243.216 g/mol
2 G_PER_L CHEBI:63037
triammonium citrate
 Role: Nitrogen source; Properties: Defined component, Simple component, Organic compound
7 CH3COONa.3H2O 5 G_PER_L Role: Buffer; Properties: Defined component, Simple component, Organic compound
8 glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Simple component, Organic compound, Defined component
9 Oxoid peptone 10 G_PER_L Properties: Undefined component
10 Lab-Lemco (Oxoid) 10 G_PER_L Properties: Undefined component
11 Yeast extract (Difco or Oxoid) 5 G_PER_L Properties: Complex component, Undefined component

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.419344Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2950
2026-01-28T19:48:22.262238Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:06.332479Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009477
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.309058+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MRS (de Man, Rogosa, Sharpe) medium" to "mrs_de_man_rogosa_sharpe_medium" for consistent matching
2026-06-05T07:41:38.470276+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:27.302995+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:09.188466+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.