Overview
- External ontology
- TOGO:M2964 — SP-4 medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2964
Composition
flowchart LR
CultureMech_009489["sp_4_medium"]
ing0_De_ionized_water["De-ionized water"]
CultureMech_009489 --> ing0_De_ionized_water
ing1_Glucose["Glucose"]
CultureMech_009489 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_Bacto_Tryptone["Bacto Tryptone"]
CultureMech_009489 --> ing2_Bacto_Tryptone
ing3_Bacto_Peptone["Bacto Peptone"]
CultureMech_009489 --> ing3_Bacto_Peptone
ing4_Mycoplasma_Broth_Base["Mycoplasma Broth Base"]
CultureMech_009489 --> ing4_Mycoplasma_Broth_Base
ing5_Fetal_bovine_serum__heated_56_C__I_hour_["Fetal bovine serum (heated 56°C- I hour)"]
CultureMech_009489 --> ing5_Fetal_bovine_serum__heated_56_C__I_hour_
ing6_Phenol_red__0_1__aqueous_["Phenol red (0.1% aqueous)"]
CultureMech_009489 --> ing6_Phenol_red__0_1__aqueous_
ing7_Penicillin_G__100_000_units_ml_["Penicillin G (100,000 units/ml)"]
CultureMech_009489 --> ing7_Penicillin_G__100_000_units_ml_
ing8_Polymyxin_B__100_000_units_ml___optional_["Polymyxin B (100,000 units/ml) (optional)"]
CultureMech_009489 --> ing8_Polymyxin_B__100_000_units_ml___optional_
ing9_CMRL_1066_tissue_culture_supplement___10x__with_glutamine_["CMRL 1066 tissue culture supplement- (10x)(with glutamine)"]
CultureMech_009489 --> ing9_CMRL_1066_tissue_culture_supplement___10x__with_glutamine_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | De-ionized water | 560 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component | |
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 3 | Bacto Tryptone | 10 G_PER_L | Properties: Undefined component | |
| 4 | Bacto Peptone | 5.3 G_PER_L | Properties: Undefined component | |
| 5 | Mycoplasma Broth Base | 3.5 G_PER_L | Properties: Complex component, Undefined component | |
| 6 | Fetal bovine serum (heated 56°C- I hour) | 170 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 7 | Phenol red (0.1% aqueous) | 20 G_PER_L | Role: pH indicator; Properties: Organic compound, Defined component, Solution | |
| 8 | Penicillin G (100,000 units/ml) | 10 G_PER_L | Role: Antimicrobial agent; Properties: Simple component, Defined component | |
| 9 | Polymyxin B (100,000 units/ml) (optional) | 5 G_PER_L | Role: Antimicrobial agent | |
| 10 | CMRL 1066 tissue culture supplement- (10x)(with glutamine) | 50 G_PER_L | Properties: Defined component, Complex component |
Solutions
Yeastolate (2% solution) (Difco)
Dose: 100 G_PER_L
Role: Nutrient source; Properties: Undefined component
Fresh yeast extract (25% solution)
Dose: 35 G_PER_L
Properties: Undefined component
Thallium acetate (1:50 solution)
Dose: 50 G_PER_L
Properties: Defined component, Simple component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sp_4_medium (CultureMech:009489)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.429215Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2964 |
| 2026-01-28T19:48:20.676247Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:34.278763+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:20:06.375226Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009489 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.359793+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SP-4 medium" to "sp_4_medium" for consistent matching |
| 2026-05-16T09:04:31.927617+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:46.137367+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:55.985008+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b0996e69aa686ed0752a6cffbdc6176e34ddf306536d0ee69251179072b63a18 |