sp_4_medium

Overview

External ontology

TOGO:M2964 — SP-4 medium

Applications

• Microbial cultivation

pH range

{'min': 7.4, 'max': 7.6}

Notes

TOGO M2964 defines SP-4 medium with 560 ml De-ionized water, 5 g Glucose, 10 g Bacto Tryptone, 5.3 g Bacto Peptone, 3.5 g Mycoplasma Broth Base, 170 ml heat-treated Fetal bovine serum, 20 ml 0.1% Phenol red, 100 ml 2% Difco Yeastolate, 10 ml Penicillin G at 100000 units/ml, optional 5 ml Polymyxin B at 100000 units/ml, 35 ml Fresh yeast extract at 25%, 50 ml 1:50 Thallium acetate, and 50 ml CMRL 1066 tissue culture supplement. The TOGO comments direct adjusting to pH 7.5-7.6, sterilizing at 121 C for 15 minutes, and record a final pH of 7.4-7.5.

Composition

flowchart LR
    CultureMech_009489["sp_4_medium"]
    ing0_De_ionized_water["De-ionized water"]
    CultureMech_009489 --> ing0_De_ionized_water
    CHEBI_15377["CHEBI:15377"]
    ing0_De_ionized_water -.-> CHEBI_15377
    ing1_Glucose["Glucose"]
    CultureMech_009489 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_Bacto_Tryptone["Bacto Tryptone"]
    CultureMech_009489 --> ing2_Bacto_Tryptone
    ing3_Bacto_Peptone["Bacto Peptone"]
    CultureMech_009489 --> ing3_Bacto_Peptone
    ing4_Mycoplasma_Broth_Base["Mycoplasma Broth Base"]
    CultureMech_009489 --> ing4_Mycoplasma_Broth_Base
    ing5_Fetal_bovine_serum__heated_56_C__I_hour_["Fetal bovine serum (heated 56°C- I hour)"]
    CultureMech_009489 --> ing5_Fetal_bovine_serum__heated_56_C__I_hour_
    ing6_CMRL_1066_tissue_culture_supplement___10x__with_glutamine_["CMRL 1066 tissue culture supplement- (10x)(with glutamine)"]
    CultureMech_009489 --> ing6_CMRL_1066_tissue_culture_supplement___10x__with_glutamine_

Ingredients (7)

Preparation

1. Mix De-ionized water, Glucose, Bacto Tryptone, Bacto Peptone, and Mycoplasma Broth Base as the basal medium.
2. Adjust the basal medium to pH 7.5-7.6.
3. Sterilize the basal medium at 121 C for 15 minutes.
4. Add fetal bovine serum, Phenol red, Yeastolate, Penicillin G, optional Polymyxin B, Fresh yeast extract, Thallium acetate, and CMRL 1066 tissue culture supplement.

Solutions

Target organisms

No target organisms are recorded.

Provenance