Overview
- External ontology
- TOGO:M3010 — PYG Medium (K)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3010 Original source: JCM - JCM_M1338 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1338
Composition
flowchart LR
CultureMech_009528["pyg_medium_k"]
ing0_Distilled_water["Distilled water"]
CultureMech_009528 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009528 --> ing1_Yeast_extract
ing2_KH2PO4["KH2PO4"]
CultureMech_009528 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Resazurin["Resazurin"]
CultureMech_009528 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_Trypticase_peptone["Trypticase peptone"]
CultureMech_009528 --> ing4_Trypticase_peptone
ing5_L_Cysteine_HCl_H2O["L-Cysteine·HCl·H2O"]
CultureMech_009528 --> ing5_L_Cysteine_HCl_H2O
ing6_Peptone["Peptone"]
CultureMech_009528 --> ing6_Peptone
ing7_N2["N2"]
CultureMech_009528 --> ing7_N2
CHEBI_17997["CHEBI:17997"]
ing7_N2 -.-> CHEBI_17997
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
940 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 | Yeast extract | 10 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component |
| 5 | Trypticase peptone | 5 G_PER_L | Properties: Undefined component | |
| 6 | L-Cysteine·HCl·H2O | 0.5 G_PER_L | Properties: Defined component, Simple component, Organic compound | |
| 7 | Peptone | 5 G_PER_L | ||
| 8 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
Salt solution (see Medium [M695])
Dose: 40 G_PER_L
Cross-reference to Medium M695
Hemin solution (see Medium [M470])
Dose: 10 G_PER_L
Cross-reference to Medium M470
Menadione solution (see Medium [M470])
Dose: 10 G_PER_L
Cross-reference to Medium M470
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pyg_medium_k (CultureMech:009528)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.680630Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M3010 |
| 2026-01-28T19:48:11.892399Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:19.627510+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:20:06.611917Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009528 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.626534+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYG Medium (K)" to "pyg_medium_k" for consistent matching |
| 2026-05-16T09:04:29.834529+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:43.098368+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.601051+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.956720+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d41633c49eac85340bed5f43848b84eb436246f40b87b5af38eabecfd19d7759 |