pyg_medium_k

Overview

External ontology

TOGO:M3010 — PYG Medium (K)

Applications

• Microbial cultivation

pH range

{'min': 7.0, 'max': 7.2}

Notes

TOGO M3010 imports JCM Medium 1338 PYG Medium (K). JCM Medium 1338 lists 5 g trypticase peptone, 5 g peptone, 10 g yeast extract, 2 g KH2PO4, 0.5 g L-Cysteine HCl H2O, 1 mg resazurin, 40 ml salt solution from JCM Medium 676, and 940 ml distilled water, then instructs anaerobic addition of 10 ml hemin solution and 10 ml menadione solution from JCM Medium 469 after autoclaving under N2.

Composition

flowchart LR
    CultureMech_009528["pyg_medium_k"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_009528 --> ing0_Trypticase_peptone
    ing1_Peptone["Peptone"]
    CultureMech_009528 --> ing1_Peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_009528 --> ing2_Yeast_extract
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009528 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_L_Cysteine_x_HCl_x_H2O["L-Cysteine x HCl x H2O"]
    CultureMech_009528 --> ing4_L_Cysteine_x_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing4_L_Cysteine_x_HCl_x_H2O -.-> CHEBI_91248
    ing5_Resazurin["Resazurin"]
    CultureMech_009528 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_Distilled_water["Distilled water"]
    CultureMech_009528 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377
    ing7_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009528 --> ing7_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing7_Nitrogen_gas -.-> CHEBI_17997

Ingredients (8)

Preparation

1. Mix the basal PYG Medium (K) components thoroughly.
2. Autoclave the basal medium under a nitrogen atmosphere.
3. Cool the autoclaved basal medium.
4. Aseptically and anaerobically add autoclaved hemin solution and menadione solution.
5. Adjust pH to 7.0-7.2, if necessary.

Solutions

Target organisms

No target organisms are recorded.

Provenance