Overview
- External ontology
- TOGO:M3029 — Ectothiorhodospira Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M3029 Original source: JCM - JCM_M1386 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1386
Composition
flowchart LR
CultureMech_009540["ectothiorhodospira_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009540 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009540 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009540 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_009540 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_009540 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_009540 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_Na2CO3["Na2CO3"]
CultureMech_009540 --> ing6_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing6_Na2CO3 -.-> CHEBI_29377
ing7_Na2SO4["Na2SO4"]
CultureMech_009540 --> ing7_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing7_Na2SO4 -.-> CHEBI_32149
ing8_Sodium_succinate["Sodium succinate"]
CultureMech_009540 --> ing8_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing8_Sodium_succinate -.-> CHEBI_63675
ing9_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009540 --> ing9_Yeast_extract__BD_Difco_
ing10_N2["N2"]
CultureMech_009540 --> ing10_N2
CHEBI_17997["CHEBI:17997"]
ing10_N2 -.-> CHEBI_17997
ing11_NaHCO3["NaHCO3"]
CultureMech_009540 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Na2S_9H2O["Na2S·9H2O"]
CultureMech_009540 --> ing12_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_9H2O -.-> CHEBI_76209
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1100.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 3 duplicates: 800.0, 200.0, 100.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
180 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.8 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
Na2CO3
CO3.2Na 105.988 g/mol
|
6 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
Na2SO4
2Na.O4S 142.043 g/mol
|
20 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 9 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
1 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
Role: Growth factor; Properties: Defined component, Simple component, Organic compound |
| 10 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 11 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
14 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 13 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Simple component, Inorganic compound, Defined component |
Solutions
Trace element solution SLA (see Medium [M352])
Dose: 1 G_PER_L
Cross-reference to Medium M352
Vitamin solution VA (see Medium [M352])
Dose: 1 G_PER_L
Cross-reference to Medium M352
Bicarbonate solution
Dose: 200 G_PER_L
See mediadive_29_Bicarbonate_solution.yaml for composition
Na2S·9H2O solution
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- ectothiorhodospira_medium (CultureMech:009540)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.699324Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M3029 |
| 2026-01-28T19:48:17.939657Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:29.707142+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:08:58.595860Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:06.705763Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009540 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.727155+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ectothiorhodospira Medium" to "ectothiorhodospira_medium" for consistent matching |
| 2026-05-16T09:03:31.305654+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:59.334819+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.626776+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:33.374535+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7218f8865f823ef25b7b20449fe090283e2fb7279ac043d965cc980a7806bb5d |