ectothiorhodospira_medium

CultureMech:009540 bacterial COMPLEX LIQUID
Original name: Ectothiorhodospira Medium

Overview

External ontology
TOGO:M3029 — Ectothiorhodospira Medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M3029 Original source: JCM - JCM_M1386 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1386

Composition

flowchart LR
    CultureMech_009540["ectothiorhodospira_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009540 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_009540 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_009540 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009540 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_009540 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_009540 --> ing5_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_6H2O -.-> CHEBI_86345
    ing6_Na2CO3["Na2CO3"]
    CultureMech_009540 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377
    ing7_Na2SO4["Na2SO4"]
    CultureMech_009540 --> ing7_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing7_Na2SO4 -.-> CHEBI_32149
    ing8_Sodium_succinate["Sodium succinate"]
    CultureMech_009540 --> ing8_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing8_Sodium_succinate -.-> CHEBI_63675
    ing9_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009540 --> ing9_Yeast_extract__BD_Difco_
    ing10_N2["N2"]
    CultureMech_009540 --> ing10_N2
    CHEBI_17997["CHEBI:17997"]
    ing10_N2 -.-> CHEBI_17997
    ing11_NaHCO3["NaHCO3"]
    CultureMech_009540 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_Na2S_9H2O["Na2S·9H2O"]
    CultureMech_009540 --> ing12_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_9H2O -.-> CHEBI_76209

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1100.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 3 duplicates: 800.0, 200.0, 100.0]
2 NaCl
Cl.Na 58.443 g/mol
180 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
5 NH4Cl
Cl.H4N 53.492 g/mol
0.8 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
6 MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
7 Na2CO3
CO3.2Na 105.988 g/mol
6 G_PER_L CHEBI:29377
sodium carbonate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
8 Na2SO4
2Na.O4S 142.043 g/mol
20 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
9 Sodium succinate
C4H4O4.2Na 162.052 g/mol
1 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
 Role: Growth factor; Properties: Defined component, Simple component, Organic compound
10 Yeast extract (BD-Difco) 0.5 G_PER_L Properties: Undefined component
11 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
12 NaHCO3
CHO3.Na 84.006 g/mol
14 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
13 Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Simple component, Inorganic compound, Defined component

Solutions

Trace element solution SLA (see Medium [M352])

Dose: 1 G_PER_L

Cross-reference to Medium M352

Vitamin solution VA (see Medium [M352])

Dose: 1 G_PER_L

Cross-reference to Medium M352

Bicarbonate solution

Dose: 200 G_PER_L

See mediadive_29_Bicarbonate_solution.yaml for composition

Na2S·9H2O solution

Dose: 10 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:53.699324Z togo-import Imported from TOGO Medium Source: JCM, ID: M3029
2026-01-28T19:48:17.939657Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:29.707142+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 4 solution(s) from ingredients
2026-03-13T03:08:58.595860Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:06.705763Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009540
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:03.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.727155+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Ectothiorhodospira Medium" to "ectothiorhodospira_medium" for consistent matching
2026-05-16T09:03:31.305654+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:59.334819+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.626776+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.