Overview
- External ontology
- TOGO:M3138 — Modified Methanobacterium Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M3138 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1523.pdf Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009606["modified_methanobacterium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009606 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009606 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009606 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009606 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009606 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_009606 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_009606 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_NaHCO3["NaHCO3"]
CultureMech_009606 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Na_acetate["Na-acetate"]
CultureMech_009606 --> ing8_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing8_Na_acetate -.-> CHEBI_32954
ing9_Na_formate["Na-formate"]
CultureMech_009606 --> ing9_Na_formate
CHEBI_62965["CHEBI:62965"]
ing9_Na_formate -.-> CHEBI_62965
ing10_Calf_brains["Calf brains"]
CultureMech_009606 --> ing10_Calf_brains
ing11_Beef_heart["Beef heart"]
CultureMech_009606 --> ing11_Beef_heart
ing12_Proteose_peptone["Proteose peptone"]
CultureMech_009606 --> ing12_Proteose_peptone
ing13_Dextrose["Dextrose"]
CultureMech_009606 --> ing13_Dextrose
CHEBI_17634["CHEBI:17634"]
ing13_Dextrose -.-> CHEBI_17634
ing14_Sodium_chloride["Sodium chloride"]
CultureMech_009606 --> ing14_Sodium_chloride
ing14_Sodium_chloride -.-> CHEBI_26710
ing15_Disodium_phosphate["Disodium phosphate"]
CultureMech_009606 --> ing15_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing15_Disodium_phosphate -.-> CHEBI_34683
ing16_L_Cysteine_HCl_x_H2O["L-Cysteine-HCl x H2O"]
CultureMech_009606 --> ing16_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing16_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing17_Proteose_peptone__BD_Difco_["Proteose peptone (BD Difco)"]
CultureMech_009606 --> ing17_Proteose_peptone__BD_Difco_
ing18_Yeast_extract__OXOID_["Yeast extract (OXOID)"]
CultureMech_009606 --> ing18_Yeast_extract__OXOID_
ing19_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009606 --> ing19_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing19_Carbon_dioxide_gas -.-> CHEBI_16526
ing20_N2_gas["N2 gas"]
CultureMech_009606 --> ing20_N2_gas
ing21_Hydrogen_gas["Hydrogen gas"]
CultureMech_009606 --> ing21_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing21_Hydrogen_gas -.-> CHEBI_18276
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009606 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_H3BO3["H3BO3"]
CultureMech_009606 --> ing23_H3BO3
CHEBI_33118["CHEBI:33118"]
ing23_H3BO3 -.-> CHEBI_33118
ing24_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_009606 --> ing24_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing24_MnCl2_x_4_H2O -.-> CHEBI_86368
ing25_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009606 --> ing25_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing25_CoCl2_x_6_H2O -.-> CHEBI_53503
ing26_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009606 --> ing26_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing26_NiCl2_x_6_H2O -.-> CHEBI_34887
ing27_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_009606 --> ing27_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing27_CuCl2_x_2_H2O -.-> CHEBI_86318
ing28_ZnCl2["ZnCl2"]
CultureMech_009606 --> ing28_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing28_ZnCl2 -.-> CHEBI_49976
ing29_HCl__25___7_7_M_["HCl (25%; 7.7 M)"]
CultureMech_009606 --> ing29_HCl__25___7_7_M_
CultureMech_009606 --> more["...8 more"]
Ingredients (38)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2990.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1000.0, 990.0, 1000.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.4 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.4 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 10 |
Na-formate
CHO2.Na 68.007 g/mol
|
2 G_PER_L |
CHEBI:62965
sodium formate
|
Properties: Defined component, Inorganic compound, Simple component |
| 11 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 12 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 13 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 14 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 15 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 16 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 17 |
L-Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Organic compound, Simple component, Defined component |
| 18 | Proteose peptone (BD Difco) | 6 G_PER_L | Properties: Undefined component | |
| 19 | Yeast extract (OXOID) | 2 G_PER_L | Properties: Undefined component, Complex component | |
| 20 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 21 | N2 gas | variable VARIABLE | Properties: ガス | |
| 22 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
H3BO3
H3BO3 61.833 g/mol
|
6 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
190 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 | HCl (25%; 7.7 M) | 10 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Solution | |
| 31 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
80 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
100 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
300 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 35 | Vitamin B12 | 100 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 36 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
200 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 37 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
200 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 38 |
D(+)-Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Trace element solution SL-10
Dose: 1 G_PER_L
See mediadive_4178_Trace_element_solution_SL-10.yaml for composition
Seven vitamins solution
Dose: 1 G_PER_L
See mediadive_4611_Seven_vitamins_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M3138_Modified_Methanobacterium_Medium (CultureMech:009606)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.509521Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3138 |
| 2026-01-28T19:48:15.866140Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:04:26.161600+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:54.716689Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:07.396378Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009606 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.424344+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Methanobacterium Medium" to "modified_methanobacterium_medium" for consistent matching |
| 2026-05-16T09:03:16.828323+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:40.100429+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:29.456568+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.195878+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:27.375487+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6e3500bd492fe03aeedd2d4fcbd7110fabca10003db6d26b3ee06f95aa940159 |