modified_methanobacterium_medium (CultureMech:009606)

Overview

External ontology

TOGO:M3138 — Modified Methanobacterium Medium

Applications

Microbial cultivation

High-metal flag

True

Notes

Source: https://togomedium.org/medium/M3138 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1523.pdf Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_009606["modified_methanobacterium_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009606 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_009606 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_009606 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_009606 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_009606 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_009606 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_009606 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_NaHCO3["NaHCO3"]
    CultureMech_009606 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Na_acetate["Na-acetate"]
    CultureMech_009606 --> ing8_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing8_Na_acetate -.-> CHEBI_32954
    ing9_Na_formate["Na-formate"]
    CultureMech_009606 --> ing9_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing9_Na_formate -.-> CHEBI_62965
    ing10_Calf_brains["Calf brains"]
    CultureMech_009606 --> ing10_Calf_brains
    ing11_Beef_heart["Beef heart"]
    CultureMech_009606 --> ing11_Beef_heart
    ing12_Proteose_peptone["Proteose peptone"]
    CultureMech_009606 --> ing12_Proteose_peptone
    ing13_Dextrose["Dextrose"]
    CultureMech_009606 --> ing13_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing13_Dextrose -.-> CHEBI_17634
    ing14_Sodium_chloride["Sodium chloride"]
    CultureMech_009606 --> ing14_Sodium_chloride
    ing14_Sodium_chloride -.-> CHEBI_26710
    ing15_Disodium_phosphate["Disodium phosphate"]
    CultureMech_009606 --> ing15_Disodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing15_Disodium_phosphate -.-> CHEBI_34683
    ing16_L_Cysteine_HCl_x_H2O["L-Cysteine-HCl x H2O"]
    CultureMech_009606 --> ing16_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing16_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing17_Proteose_peptone__BD_Difco_["Proteose peptone (BD Difco)"]
    CultureMech_009606 --> ing17_Proteose_peptone__BD_Difco_
    ing18_Yeast_extract__OXOID_["Yeast extract (OXOID)"]
    CultureMech_009606 --> ing18_Yeast_extract__OXOID_
    ing19_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009606 --> ing19_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing19_Carbon_dioxide_gas -.-> CHEBI_16526
    ing20_N2_gas["N2 gas"]
    CultureMech_009606 --> ing20_N2_gas
    ing21_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009606 --> ing21_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing21_Hydrogen_gas -.-> CHEBI_18276
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_009606 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing23_H3BO3["H3BO3"]
    CultureMech_009606 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_009606 --> ing24_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing24_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing25_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_009606 --> ing25_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing25_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing26_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_009606 --> ing26_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing26_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing27_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_009606 --> ing27_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing27_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing28_ZnCl2["ZnCl2"]
    CultureMech_009606 --> ing28_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing28_ZnCl2 -.-> CHEBI_49976
    ing29_HCl__25___7_7_M_["HCl (25%; 7.7 M)"]
    CultureMech_009606 --> ing29_HCl__25___7_7_M_
    CultureMech_009606 --> more["...8 more"]

Ingredients (38)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	2990.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1000.0, 990.0, 1000.0]
2	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.4 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3	NaCl Cl.Na 58.443 g/mol	0.4 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.05 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5	KH2PO4 H2O4P.K 136.084 g/mol	0.5 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6	NH4Cl Cl.H4N 53.492 g/mol	0.4 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
7	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.5 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
8	NaHCO3 CHO3.Na 84.006 g/mol	4 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
9	Na-acetate C2H3O2.Na 82.034 g/mol	1 G_PER_L	CHEBI:32954 sodium acetate	Role: Buffer; Properties: Organic compound, Defined component, Simple component
10	Na-formate CHO2.Na 68.007 g/mol	2 G_PER_L	CHEBI:62965 sodium formate	Properties: Defined component, Inorganic compound, Simple component
11	Calf brains Difco/BD Brain Heart Infusion 237500	variable VARIABLE	UBERON:0000955 brain	infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product
12	Beef heart Difco/BD Brain Heart Infusion 237500	variable VARIABLE	UBERON:0000948 heart	infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product
13	Proteose peptone Difco/BD Brain Heart Infusion 237500	10.0 G_PER_L	MICRO:0000180 (source link unavailable) proteose peptone	Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product
14	Dextrose C6H12O6 180.156 g/mol Difco/BD Brain Heart Infusion 237500	2.0 G_PER_L	CHEBI:17634 D-glucose	Carbon/energy source; Constituent of Brain Heart Infusion commercial product
15	Sodium chloride Cl.Na 58.443 g/mol Difco/BD Brain Heart Infusion 237500	5.0 G_PER_L	CHEBI:26710 sodium chloride	Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product
16	Disodium phosphate HO4P.2Na 141.958 g/mol Difco/BD Brain Heart Infusion 237500	2.5 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product
17	L-Cysteine-HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.5 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	Properties: Organic compound, Simple component, Defined component
18	Proteose peptone (BD Difco)	6 G_PER_L		Properties: Undefined component
19	Yeast extract (OXOID)	2 G_PER_L		Properties: Undefined component, Complex component
20	Carbon dioxide gas CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Properties: ガス
21	N2 gas	variable VARIABLE		Properties: ガス
22	Hydrogen gas H2 2.016 g/mol	variable VARIABLE	CHEBI:18276 dihydrogen	Properties: ガス
23	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	36 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
24	H3BO3 H3BO3 61.833 g/mol	6 G_PER_L	CHEBI:33118 boric acid	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
25	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	100 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
26	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	190 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
27	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	24 G_PER_L	CHEBI:34887 nickel dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
28	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	2 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
29	ZnCl2 2Cl.Zn 136.296 g/mol	70 G_PER_L	CHEBI:49976 zinc dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
30	HCl (25%; 7.7 M)	10 G_PER_L		Role: Solvating media; Properties: Inorganic compound, Defined component, Solution
31	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	1.5 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
32	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	80 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
33	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	100 G_PER_L	CHEBI:31345 Calcium pantothenate	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
34	Pyridoxine hydrochloride C8H11NO3.HCl 205.641 g/mol	300 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
35	Vitamin B12	100 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
36	Nicotinic acid C6H5NO2 123.111 g/mol	200 G_PER_L	CHEBI:15940 nicotinic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
37	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	200 G_PER_L	CHEBI:132751 thiamine hydrochloride dihydrate	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
38	D(+)-Biotin C10H16N2O3S 244.316 g/mol	20 G_PER_L	CHEBI:15956 biotin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component

Solutions

Na-resazurin solution (0.1% w/v)

Dose: 0.5 G_PER_L

Role: pH dependent redox indicator; Properties: Defined component, Solution

Trace element solution SL-10

Dose: 1 G_PER_L

See mediadive_4178_Trace_element_solution_SL-10.yaml for composition

Seven vitamins solution

Dose: 1 G_PER_L

See mediadive_4611_Seven_vitamins_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:57.509521Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M3138
2026-01-28T19:48:15.866140Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: N2 gas
2026-03-09T08:04:26.161600+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 3 solution(s) from ingredients
2026-03-13T03:08:54.716689Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 3 duplicate ingredient(s)
2026-03-13T06:20:07.396378Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009606
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:59.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:26.424344+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Modified Methanobacterium Medium" to "modified_methanobacterium_medium" for consistent matching
2026-05-16T09:03:16.828323+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:40.100429+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:29.456568+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:50.195878+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.