modified_p7_medium

CultureMech:009620 bacterial COMPLEX LIQUID
Original name: Modified P7 medium

Overview

External ontology
TOGO:M3161 — Modified P7 medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M3161

Composition

flowchart LR
    CultureMech_009620["modified_p7_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_009620 --> ing0_Yeast_extract
    ing1_Morpholinoethanesulfonic_acid__MES_["Morpholinoethanesulfonic acid (MES)"]
    CultureMech_009620 --> ing1_Morpholinoethanesulfonic_acid__MES_
    ing2_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009620 --> ing2_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing2_Carbon_dioxide_gas -.-> CHEBI_16526
    ing3_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009620 --> ing3_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing3_Hydrogen_gas -.-> CHEBI_18276
    ing4_Carbon_monoxide["Carbon monoxide"]
    CultureMech_009620 --> ing4_Carbon_monoxide
    ing5_Distilled_water["Distilled water"]
    CultureMech_009620 --> ing5_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Distilled_water -.-> CHEBI_15377
    ing6_Sodium_chloride["Sodium chloride"]
    CultureMech_009620 --> ing6_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing6_Sodium_chloride -.-> CHEBI_26710
    ing7_Potassium_monophosphate["Potassium monophosphate"]
    CultureMech_009620 --> ing7_Potassium_monophosphate
    CHEBI_131527["CHEBI:131527"]
    ing7_Potassium_monophosphate -.-> CHEBI_131527
    ing8_Ammonium_chloride["Ammonium chloride"]
    CultureMech_009620 --> ing8_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing8_Ammonium_chloride -.-> CHEBI_31206
    ing9_Potassium_chloride["Potassium chloride"]
    CultureMech_009620 --> ing9_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing9_Potassium_chloride -.-> CHEBI_32588
    ing10_Calcium_chloride["Calcium chloride"]
    CultureMech_009620 --> ing10_Calcium_chloride
    CHEBI_86158["CHEBI:86158"]
    ing10_Calcium_chloride -.-> CHEBI_86158
    ing11_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_009620 --> ing11_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing11_Magnesium_sulfate -.-> CHEBI_32599
    ing12_Paraamino_benzoic_acid["Paraamino benzoic acid"]
    CultureMech_009620 --> ing12_Paraamino_benzoic_acid
    ing13_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_009620 --> ing13_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing13_Calcium_pantothenate -.-> CHEBI_31345
    ing14_Folic_acid["Folic acid"]
    CultureMech_009620 --> ing14_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing14_Folic_acid -.-> CHEBI_27470
    ing15_Vitamin_B12["Vitamin B12"]
    CultureMech_009620 --> ing15_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing15_Vitamin_B12 -.-> CHEBI_176843
    ing16_RibofIavin["RibofIavin"]
    CultureMech_009620 --> ing16_RibofIavin
    ing17_Nicotinic_acid["Nicotinic acid"]
    CultureMech_009620 --> ing17_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing17_Nicotinic_acid -.-> CHEBI_15940
    ing18_2_Mercaptoethanesulfonic_acid["2-Mercaptoethanesulfonic acid"]
    CultureMech_009620 --> ing18_2_Mercaptoethanesulfonic_acid
    CHEBI_17905["CHEBI:17905"]
    ing18_2_Mercaptoethanesulfonic_acid -.-> CHEBI_17905
    ing19_Thiamine["Thiamine"]
    CultureMech_009620 --> ing19_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing19_Thiamine -.-> CHEBI_18385
    ing20_Pyridoxine["Pyridoxine"]
    CultureMech_009620 --> ing20_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing20_Pyridoxine -.-> CHEBI_16709
    ing21_Thioctic_acid["Thioctic acid"]
    CultureMech_009620 --> ing21_Thioctic_acid
    CHEBI_30314["CHEBI:30314"]
    ing21_Thioctic_acid -.-> CHEBI_30314
    ing22_d_Biotin["d-Biotin"]
    CultureMech_009620 --> ing22_d_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing22_d_Biotin -.-> CHEBI_15956
    ing23_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_009620 --> ing23_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing23_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing24_Sodium_tungstate["Sodium tungstate"]
    CultureMech_009620 --> ing24_Sodium_tungstate
    CHEBI_63940["CHEBI:63940"]
    ing24_Sodium_tungstate -.-> CHEBI_63940
    ing25_Cobalt_chloride["Cobalt chloride"]
    CultureMech_009620 --> ing25_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing25_Cobalt_chloride -.-> CHEBI_35696
    ing26_Cupric_chloride["Cupric chloride"]
    CultureMech_009620 --> ing26_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing26_Cupric_chloride -.-> CHEBI_49553
    ing27_Sodium_selenate["Sodium selenate"]
    CultureMech_009620 --> ing27_Sodium_selenate
    CHEBI_77775["CHEBI:77775"]
    ing27_Sodium_selenate -.-> CHEBI_77775
    ing28_Nickel_chloride["Nickel chloride"]
    CultureMech_009620 --> ing28_Nickel_chloride
    CHEBI_34887["CHEBI:34887"]
    ing28_Nickel_chloride -.-> CHEBI_34887
    ing29_Sodium_molybdate["Sodium molybdate"]
    CultureMech_009620 --> ing29_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing29_Sodium_molybdate -.-> CHEBI_75215
    CultureMech_009620 --> more["...3 more"]

Ingredients (33)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.5 G_PER_L Role: Nutrient source; Properties: Undefined component
2 Morpholinoethanesulfonic acid (MES) 5 G_PER_L Role: Buffer; Properties: Organic compound, Defined component, Simple component
3 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
4 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス
5 Carbon monoxide variable VARIABLE Properties: ガス
6 Distilled water
H2O 18.015 g/mol
3.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0]
7 Sodium chloride
Cl.Na 58.443 g/mol
80 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 Potassium monophosphate
HO4P.2K 174.174 g/mol
10 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
9 Ammonium chloride
Cl.H4N 53.492 g/mol
100 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
10 Potassium chloride
Cl.K 74.551 g/mol
10 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 Calcium chloride
Ca.2Cl.2H2O 147.014 g/mol
4 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12 Magnesium sulfate
Mg.O4S 120.368 g/mol
20 G_PER_L CHEBI:32599
magnesium sulfate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 Paraamino benzoic acid 5 G_PER_L Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
5 G_PER_L CHEBI:31345
Calcium pantothenate
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
15 Folic acid
C19H19N7O6 441.404 g/mol
2 G_PER_L CHEBI:27470
folic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
16 Vitamin B12 5 G_PER_L CHEBI:176843
vitamin B12
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
17 RibofIavin 5 G_PER_L Role: Vitamin; Properties: Organic compound, Defined component, Simple component
18 Nicotinic acid
C6H5NO2 123.111 g/mol
5 G_PER_L CHEBI:15940
nicotinic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
19 2-Mercaptoethanesulfonic acid
C2H6O3S2 142.201 g/mol
2 G_PER_L CHEBI:17905
coenzyme M
 Role: Growth factor; Properties: Organic compound, Defined component, Simple component
20 Thiamine
C12H17N4OS 265.362 g/mol
5 G_PER_L CHEBI:18385
thiamine(1+)
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
21 Pyridoxine
C8H11NO3 169.18 g/mol
10 G_PER_L CHEBI:16709
pyridoxine
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
22 Thioctic acid
C8H14O2S2 206.332 g/mol
5 G_PER_L CHEBI:30314
(R)-lipoic acid
 Role: Growth factor; Properties: Organic compound, Defined component, Simple component
23 d-Biotin
C10H16N2O3S 244.316 g/mol
2 G_PER_L CHEBI:15956
biotin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
24 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
2 G_PER_L CHEBI:44557
nitrilotriacetic acid
 Role: Mineral source; Properties: Organic compound, Defined component, Simple component
25 Sodium tungstate
2Na.O4W 293.816 g/mol
20 G_PER_L CHEBI:63940
sodium tungstate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
26 Cobalt chloride
2Cl.Co 129.839 g/mol
0.2 G_PER_L CHEBI:35696
cobalt dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
27 Cupric chloride
Cl2Cu 134.452 g/mol
20 G_PER_L CHEBI:49553
copper(II) chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
28 Sodium selenate
2Na.O4Se 188.936 g/mol
20 G_PER_L CHEBI:77775
sodium selenate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
29 Nickel chloride
Cl2Ni 129.599 g/mol
20 G_PER_L CHEBI:34887
nickel dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
30 Sodium molybdate
MoO4.2Na 205.916 g/mol
20 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)
 Role: Mineral source; Properties: Organic compound, Defined component, Simple component
31 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
1 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
32 Zinc sulfate
O4S.Zn 161.453 g/mol
0.2 G_PER_L CHEBI:35176
zinc sulfate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
33 Ferrous ammonium sulfate 0.8 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component

Solutions

0.1% Resazurin solution

Dose: 0.1 G_PER_L

Role: pH dependent redox indicator; Properties: Defined component, Solution

4% cysteine-sulfide solution

Dose: 5 G_PER_L

Properties: Complex component, Defined component, Solution

Mineral stock solution

Dose: 30 G_PER_L

Vitamin stock solution

Dose: 10 G_PER_L

Trace metal stock solution

Dose: 10 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.549636Z togo-import Imported from TOGO Medium Source: TOGO, ID: M3161
2026-01-28T19:48:19.970877Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Carbon monoxide
2026-03-09T08:04:33.134965+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T03:09:02.513459Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:07.600827Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009620
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:08.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:26.622887+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified P7 medium" to "modified_p7_medium" for consistent matching
2026-05-16T09:04:26.101544+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:41:37.574758+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:49.522147+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 749ec105d3718ac630dc3a55184e621e9731042eeb4e3bc800eb98109fa80336