Overview
- External ontology
- TOGO:M3161 — Modified P7 medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M3161
Composition
flowchart LR
CultureMech_009620["modified_p7_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_009620 --> ing0_Yeast_extract
ing1_Morpholinoethanesulfonic_acid__MES_["Morpholinoethanesulfonic acid (MES)"]
CultureMech_009620 --> ing1_Morpholinoethanesulfonic_acid__MES_
ing2_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009620 --> ing2_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing2_Carbon_dioxide_gas -.-> CHEBI_16526
ing3_Hydrogen_gas["Hydrogen gas"]
CultureMech_009620 --> ing3_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing3_Hydrogen_gas -.-> CHEBI_18276
ing4_Carbon_monoxide["Carbon monoxide"]
CultureMech_009620 --> ing4_Carbon_monoxide
CHEBI_17245["CHEBI:17245"]
ing4_Carbon_monoxide -.-> CHEBI_17245
ing5_Distilled_water["Distilled water"]
CultureMech_009620 --> ing5_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing5_Distilled_water -.-> CHEBI_15377
ing6_Sodium_chloride["Sodium chloride"]
CultureMech_009620 --> ing6_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing6_Sodium_chloride -.-> CHEBI_26710
ing7_Potassium_monophosphate["Potassium monophosphate"]
CultureMech_009620 --> ing7_Potassium_monophosphate
CHEBI_131527["CHEBI:131527"]
ing7_Potassium_monophosphate -.-> CHEBI_131527
ing8_Ammonium_chloride["Ammonium chloride"]
CultureMech_009620 --> ing8_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing8_Ammonium_chloride -.-> CHEBI_31206
ing9_Potassium_chloride["Potassium chloride"]
CultureMech_009620 --> ing9_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing9_Potassium_chloride -.-> CHEBI_32588
ing10_Calcium_chloride["Calcium chloride"]
CultureMech_009620 --> ing10_Calcium_chloride
CHEBI_86158["CHEBI:86158"]
ing10_Calcium_chloride -.-> CHEBI_86158
ing11_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_009620 --> ing11_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing11_Magnesium_sulfate -.-> CHEBI_32599
ing12_Paraamino_benzoic_acid["Paraamino benzoic acid"]
CultureMech_009620 --> ing12_Paraamino_benzoic_acid
ing13_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_009620 --> ing13_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing13_Calcium_pantothenate -.-> CHEBI_31345
ing14_Folic_acid["Folic acid"]
CultureMech_009620 --> ing14_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing14_Folic_acid -.-> CHEBI_27470
ing15_Vitamin_B12["Vitamin B12"]
CultureMech_009620 --> ing15_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing15_Vitamin_B12 -.-> CHEBI_176843
ing16_RibofIavin["RibofIavin"]
CultureMech_009620 --> ing16_RibofIavin
ing17_Nicotinic_acid["Nicotinic acid"]
CultureMech_009620 --> ing17_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing17_Nicotinic_acid -.-> CHEBI_15940
ing18_2_Mercaptoethanesulfonic_acid["2-Mercaptoethanesulfonic acid"]
CultureMech_009620 --> ing18_2_Mercaptoethanesulfonic_acid
CHEBI_17905["CHEBI:17905"]
ing18_2_Mercaptoethanesulfonic_acid -.-> CHEBI_17905
ing19_Thiamine["Thiamine"]
CultureMech_009620 --> ing19_Thiamine
CHEBI_18385["CHEBI:18385"]
ing19_Thiamine -.-> CHEBI_18385
ing20_Pyridoxine["Pyridoxine"]
CultureMech_009620 --> ing20_Pyridoxine
CHEBI_16709["CHEBI:16709"]
ing20_Pyridoxine -.-> CHEBI_16709
ing21_Thioctic_acid["Thioctic acid"]
CultureMech_009620 --> ing21_Thioctic_acid
CHEBI_30314["CHEBI:30314"]
ing21_Thioctic_acid -.-> CHEBI_30314
ing22_d_Biotin["d-Biotin"]
CultureMech_009620 --> ing22_d_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_d_Biotin -.-> CHEBI_15956
ing23_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009620 --> ing23_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing23_Nitrilotriacetic_acid -.-> CHEBI_44557
ing24_Sodium_tungstate["Sodium tungstate"]
CultureMech_009620 --> ing24_Sodium_tungstate
CHEBI_63940["CHEBI:63940"]
ing24_Sodium_tungstate -.-> CHEBI_63940
ing25_Cobalt_chloride["Cobalt chloride"]
CultureMech_009620 --> ing25_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing25_Cobalt_chloride -.-> CHEBI_35696
ing26_Cupric_chloride["Cupric chloride"]
CultureMech_009620 --> ing26_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing26_Cupric_chloride -.-> CHEBI_49553
ing27_Sodium_selenate["Sodium selenate"]
CultureMech_009620 --> ing27_Sodium_selenate
CHEBI_77775["CHEBI:77775"]
ing27_Sodium_selenate -.-> CHEBI_77775
ing28_Nickel_chloride["Nickel chloride"]
CultureMech_009620 --> ing28_Nickel_chloride
CHEBI_34887["CHEBI:34887"]
ing28_Nickel_chloride -.-> CHEBI_34887
ing29_Sodium_molybdate["Sodium molybdate"]
CultureMech_009620 --> ing29_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing29_Sodium_molybdate -.-> CHEBI_75215
CultureMech_009620 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 | Morpholinoethanesulfonic acid (MES) | 5 G_PER_L | Role: Buffer; Properties: Organic compound, Defined component, Simple component | |
| 3 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 4 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 5 |
Carbon monoxide
CO 28.01 g/mol
|
variable VARIABLE |
CHEBI:17245
carbon monoxide
|
Properties: ガス |
| 6 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 3 identical duplicates: 1.0, 1.0, 1.0] |
| 7 |
Sodium chloride
Cl.Na 58.443 g/mol
|
80 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Potassium monophosphate
HO4P.2K 174.174 g/mol
|
10 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
100 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Potassium chloride
Cl.K 74.551 g/mol
|
10 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Calcium chloride
Ca.2Cl.2H2O 147.014 g/mol
|
4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
20 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 | Paraamino benzoic acid | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 14 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 | Vitamin B12 | 5 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 | RibofIavin | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 18 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
2-Mercaptoethanesulfonic acid
C2H6O3S2 142.201 g/mol
|
2 G_PER_L |
CHEBI:17905
coenzyme M
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 20 |
Thiamine
C12H17N4OS 265.362 g/mol
|
5 G_PER_L |
CHEBI:18385
thiamine(1+)
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Pyridoxine
C8H11NO3 169.18 g/mol
|
10 G_PER_L |
CHEBI:16709
pyridoxine
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
Thioctic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:30314
(R)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 23 |
d-Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
2 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 25 |
Sodium tungstate
2Na.O4W 293.816 g/mol
|
20 G_PER_L |
CHEBI:63940
sodium tungstate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
20 G_PER_L |
CHEBI:49553
copper(II) chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
Sodium selenate
2Na.O4Se 188.936 g/mol
|
20 G_PER_L |
CHEBI:77775
sodium selenate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
Nickel chloride
Cl2Ni 129.599 g/mol
|
20 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
20 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 31 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
1 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.2 G_PER_L |
CHEBI:35176
zinc sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 33 | Ferrous ammonium sulfate | 0.8 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
0.1% Resazurin solution
Dose: 0.1 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
4% cysteine-sulfide solution
Dose: 5 G_PER_L
Properties: Complex component, Defined component, Solution
Mineral stock solution
Dose: 30 G_PER_L
Vitamin stock solution
Dose: 10 G_PER_L
Trace metal stock solution
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.549636Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3161 |
| 2026-01-28T19:48:19.970877Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Carbon monoxide |
| 2026-03-09T08:04:33.134965+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:02.513459Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:07.600827Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009620 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.622887+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified P7 medium" to "modified_p7_medium" for consistent matching |
| 2026-05-16T09:04:26.101544+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:37.574758+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-20T19:02:56.754887+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-09-02T07:38:52.337478+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |