modified_gs_2_medium

CultureMech:009628 bacterial COMPLEX LIQUID
Original name: Modified GS-2 medium

Overview

External ontology
TOGO:M3175 — Modified GS-2 medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M3175

Composition

flowchart LR
    CultureMech_009628["modified_gs_2_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009628 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_KH2PO4["KH2PO4"]
    CultureMech_009628 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_K2HPO4["K2HPO4"]
    CultureMech_009628 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Resazurin["Resazurin"]
    CultureMech_009628 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_MOPS["MOPS"]
    CultureMech_009628 --> ing4_MOPS
    CHEBI_39074["CHEBI:39074"]
    ing4_MOPS -.-> CHEBI_39074
    ing5_L_cysteine["L-cysteine"]
    CultureMech_009628 --> ing5_L_cysteine
    CHEBI_17561["CHEBI:17561"]
    ing5_L_cysteine -.-> CHEBI_17561
    ing6_Sodium_citrate_tribasic_dihydrate["Sodium citrate tribasic dihydrate"]
    CultureMech_009628 --> ing6_Sodium_citrate_tribasic_dihydrate
    ing7_Urea["Urea"]
    CultureMech_009628 --> ing7_Urea
    CHEBI_16199["CHEBI:16199"]
    ing7_Urea -.-> CHEBI_16199
    ing8_FeSO4_7H2O["FeSO4.7H2O"]
    CultureMech_009628 --> ing8_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_7H2O -.-> CHEBI_75836
    ing9_CaCl2["CaCl2"]
    CultureMech_009628 --> ing9_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing9_CaCl2 -.-> CHEBI_3312
    ing10_MgCl2["MgCl2"]
    CultureMech_009628 --> ing10_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing10_MgCl2 -.-> CHEBI_6636
    ing11_Biotin["Biotin"]
    CultureMech_009628 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_009628 --> ing12_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_009628 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_Vitamin_B12["Vitamin B12"]
    CultureMech_009628 --> ing14_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing14_Vitamin_B12 -.-> CHEBI_176843

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
3.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0]
2 KH2PO4
H2O4P.K 136.084 g/mol
1.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
3 K2HPO4
HO4P.2K 174.174 g/mol
2.9 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
4 Resazurin
C12H7NO4 229.191 g/mol
2 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
5 MOPS 5 G_PER_L CHEBI:39074
MOPS
 Role: Buffer; Properties: Organic compound, Defined component, Simple component
6 L-cysteine
C3H7NO2S 121.161 g/mol
1 G_PER_L CHEBI:17561
L-cysteine
 Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
7 Sodium citrate tribasic dihydrate 3 G_PER_L Role: Buffer; Properties: Organic compound, Defined component, Simple component
8 Urea
CH4N2O 60.056 g/mol
2.1 G_PER_L CHEBI:16199
urea
 Properties: Defined component, Organic compound, Simple component
9 FeSO4.7H2O
Fe.7H2O.O4S 278.013 g/mol
0.125 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 CaCl2
Ca.2Cl 110.984 g/mol
11.3 G_PER_L CHEBI:3312
calcium dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 MgCl2
2Cl.Mg 95.211 g/mol
26 G_PER_L CHEBI:6636
magnesium dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12 Biotin
C10H16N2O3S 244.316 g/mol
0.2 G_PER_L CHEBI:15956
biotin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
13 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.4 G_PER_L CHEBI:30753
4-aminobenzoic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
2 G_PER_L CHEBI:30961
pyridoxine hydrochloride
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
15 Vitamin B12 0.2 G_PER_L CHEBI:176843
vitamin B12
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component

Solutions

Trace salt solution (10x)

Dose: 1 G_PER_L

Vitamin solution (100x)

Dose: 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.574632Z togo-import Imported from TOGO Medium Source: TOGO, ID: M3175
2026-01-28T19:48:37.093286Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:05:01.914085+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T03:09:33.776318Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:07.716518Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009628
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:26.729627+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified GS-2 medium" to "modified_gs_2_medium" for consistent matching
2026-05-16T09:04:25.371685+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:36.635270+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.644812+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:48.333940+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 73c19d167d45ab71b71bc3ef5e074938f949176f1dd7aa8bd9f33b7e20e9b75a