Overview
- External ontology
- TOGO:M3175 — Modified GS-2 medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3175
Composition
flowchart LR
CultureMech_009628["modified_gs_2_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009628 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_009628 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_K2HPO4["K2HPO4"]
CultureMech_009628 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Resazurin["Resazurin"]
CultureMech_009628 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_MOPS["MOPS"]
CultureMech_009628 --> ing4_MOPS
CHEBI_39074["CHEBI:39074"]
ing4_MOPS -.-> CHEBI_39074
ing5_L_cysteine["L-cysteine"]
CultureMech_009628 --> ing5_L_cysteine
CHEBI_17561["CHEBI:17561"]
ing5_L_cysteine -.-> CHEBI_17561
ing6_Sodium_citrate_tribasic_dihydrate["Sodium citrate tribasic dihydrate"]
CultureMech_009628 --> ing6_Sodium_citrate_tribasic_dihydrate
ing7_Urea["Urea"]
CultureMech_009628 --> ing7_Urea
CHEBI_16199["CHEBI:16199"]
ing7_Urea -.-> CHEBI_16199
ing8_FeSO4_7H2O["FeSO4.7H2O"]
CultureMech_009628 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
ing9_CaCl2["CaCl2"]
CultureMech_009628 --> ing9_CaCl2
CHEBI_3312["CHEBI:3312"]
ing9_CaCl2 -.-> CHEBI_3312
ing10_MgCl2["MgCl2"]
CultureMech_009628 --> ing10_MgCl2
CHEBI_6636["CHEBI:6636"]
ing10_MgCl2 -.-> CHEBI_6636
ing11_Biotin["Biotin"]
CultureMech_009628 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009628 --> ing12_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_009628 --> ing13_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing14_Vitamin_B12["Vitamin B12"]
CultureMech_009628 --> ing14_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing14_Vitamin_B12 -.-> CHEBI_176843
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 3 identical duplicates: 1.0, 1.0, 1.0] |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
2 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 5 | MOPS | 5 G_PER_L |
CHEBI:39074
MOPS
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 6 |
L-cysteine
C3H7NO2S 121.161 g/mol
|
1 G_PER_L |
CHEBI:17561
L-cysteine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 7 | Sodium citrate tribasic dihydrate | 3 G_PER_L | Role: Buffer; Properties: Organic compound, Defined component, Simple component | |
| 8 |
Urea
CH4N2O 60.056 g/mol
|
2.1 G_PER_L |
CHEBI:16199
urea
|
Properties: Defined component, Organic compound, Simple component |
| 9 |
FeSO4.7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.125 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CaCl2
Ca.2Cl 110.984 g/mol
|
11.3 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MgCl2
2Cl.Mg 95.211 g/mol
|
26 G_PER_L |
CHEBI:6636
magnesium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.4 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
2 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 | Vitamin B12 | 0.2 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Trace salt solution (10x)
Dose: 1 G_PER_L
Vitamin solution (100x)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.574632Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3175 |
| 2026-01-28T19:48:37.093286Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:01.914085+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:33.776318Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:07.716518Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009628 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.729627+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified GS-2 medium" to "modified_gs_2_medium" for consistent matching |
| 2026-05-16T09:04:25.371685+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:36.635270+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.644812+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:51.721726+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |