Overview
- External ontology
- TOGO:M3224 — LB agar medium
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': '1_3_lb_agar', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J842', 'original_category': 'bacterial'}
- {'name': '2yt_medium', 'source': 'TOGO', 'source_id': 'TOGO:M2921', 'original_category': 'bacterial'}
- {'name': 'half_concentrated_lb_luria_bertani_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1623', 'original_category': 'bacterial'}
- {'name': 'lb_luria_bertani_agar', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J443', 'original_category': 'bacterial'}
- {'name': 'lb_luria_bertani_agar_with_5_nacl', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J1240', 'original_category': 'bacterial'}
- {'name': 'lb_luria_bertani_agar_with_5_nacl_ph_9_0', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J1185', 'original_category': 'bacterial'}
- {'name': 'lb_luria_bertani_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:381', 'original_category': 'bacterial'}
- Notes
- Source: https://togomedium.org/medium/M3224 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009664["lb_agar_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_009664 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_009664 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_009664 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Agar["Agar"]
CultureMech_009664 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 3.3 G_PER_L | ||
| 2 | Yeast extract | 1.7 G_PER_L | ||
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 8 source records.
- 1_3_lb_agar (CultureMech:003187)
- 2yt_medium (CultureMech:009457)
- half_concentrated_lb_luria_bertani_medium (CultureMech:001104)
- lb_agar_medium (CultureMech:009664)
- lb_luria_bertani_agar (CultureMech:002793)
- lb_luria_bertani_agar_with_5_nacl (CultureMech:002407)
- lb_luria_bertani_agar_with_5_nacl_ph_9_0 (CultureMech:002356)
- lb_luria_bertani_medium (CultureMech:001486)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.616146Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3224 |
| 2026-01-28T19:48:18.775831Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:07.974702Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009664 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.980146+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB agar medium" to "lb_agar_medium" for consistent matching |
| 2026-06-05T07:41:10.805684+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:33.835883+00:00 | recipe-merger | Merged 8 duplicate recipes into canonical record | Sources: 1_3_lb_agar, 2yt_medium, half_concentrated_lb_luria_bertani_medium, lb_agar_medium, lb_luria_bertani_agar, lb_luria_bertani_agar_with_5_nacl, lb_luria_bertani_agar_with_5_nacl_ph_9_0, lb_luria_bertani_medium |
| 2026-08-06T03:04:33.835930+00:00 | merge_recipes.py | MERGED_RECIPES | merged 8 source recipe(s) on fingerprint 3cb7028f083ead8f828a09615b3852eeb6d919453659873c5dde37bff08442b5 |